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HIF-1α silencing suppresses growth of lung adenocarcinoma A549 cells through induction of apoptosis
- Source :
- Molecular Medicine Reports. 9:911-915
- Publication Year :
- 2014
- Publisher :
- Spandidos Publications, 2014.
-
Abstract
- Lung adenocarcinoma (AC) is one of the most deadly malignancies. The disease has a low five-year survival rate; therefore, the identification of novel therapeutic agents is required. This study aimed to investigate the effect of small interfering RNA (siRNA) targeting hypoxia‑inducible factor 1α (HIF‑1α) on the growth of AC A549 cells. A549 cells were transfected with various concentrations of HIF‑1α or control siRNA, and the effect on HIF‑1α expression was analyzed using quantitative polymerase chain reaction and western blot analysis. The effects of HIF-1α siRNA on growth inhibition and apoptosis were then assessed using standard methods. HIF‑1α siRNA treatment significantly reduced HIF‑1α mRNA and protein expression in A549 cells. Furthermore, the downregulation of HIF-1α expression inhibited the growth of A549 cells and induced apoptosis of A549 cells by upregulating caspase-3 expression. The present in vitro study demonstrates that the downregulation of HIF‑1α is capable of suppressing AC A549 cell growth, through the induction of apoptosis. This suggests that HIF‑1α inhibition may represent a promising strategy for the treatment of AC.
- Subjects :
- Cancer Research
Small interfering RNA
Lung Neoplasms
Cell
Adenocarcinoma of Lung
Apoptosis
Adenocarcinoma
Biology
Biochemistry
chemistry.chemical_compound
Downregulation and upregulation
Cell Line, Tumor
Genetics
medicine
Humans
Gene silencing
RNA, Messenger
RNA, Small Interfering
Molecular Biology
Cell Proliferation
A549 cell
Caspase 3
Transfection
Hypoxia-Inducible Factor 1, alpha Subunit
Up-Regulation
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Oncology
chemistry
Cancer research
Molecular Medicine
RNA Interference
Growth inhibition
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....f7e716fabb6fb64559f4bb322007a36f