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Regional assignment of human ESTs by whole-genome radiation hybrid mapping
- Source :
- Mammalian genome : official journal of the International Mammalian Genome Society. 7(6)
- Publication Year :
- 1996
-
Abstract
- The UK HGMP Resource Centre’s collection of human partial cDNA sequences (ESTs) have been examined for suitability for mapping by PCR on a panel of somatic cell hybrids. The chromosomal assignments of 92 ESTs were determined with a monochromosomal hybrid panel, and a subset of 45 were linked to genetic markers with a panel of whole-genome radiation hybrids (WG-RHs). These results demonstrate the potential of WG-RHs to construct a transcript map of the human genome.
- Subjects :
- Genetics
Expressed sequence tag
DNA, Complementary
Genome
Base Sequence
Molecular Sequence Data
Radiation Hybrids
Chromosome Mapping
Biology
Hybrid Cells
Polymerase Chain Reaction
Human genetics
Genetic marker
Humans
Human genome
Radiation hybrid mapping
Partial cdna
DNA Primers
Sequence Tagged Sites
Subjects
Details
- ISSN :
- 09388990
- Volume :
- 7
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Mammalian genome : official journal of the International Mammalian Genome Society
- Accession number :
- edsair.doi.dedup.....f7e1ad4f468a3710cc3c08734e32172e