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Time-gated cell imaging using long lifetime near-infrared-emitting quantum dots for autofluorescence rejection
- Source :
- Journal of Biomedical Optics, Journal of Biomedical Optics, Society of Photo-optical Instrumentation Engineers, 2014, 19 (5), pp.051208. ⟨10.1117/1.JBO.19.5.051208⟩
- Publication Year :
- 2014
- Publisher :
- SPIE-Intl Soc Optical Eng, 2014.
-
Abstract
- International audience; Fluorescence imaging is a promising technique for the detection of individual cell migration. Its sensitivity is, however, limited by a high tissue autofluorescence and a poor visible light penetration depth. In order to solve this problem, the fluorescence signal peak wavelength should lie in an absorption and diffusion free region and should be distinguishable, either spectrally or temporally, from the autofluorescence background. We present, here, the synthesis and characterization of low toxicity Zn-Cu-In-Se/ZnS core/shell quantum dots. Their fluorescence emission wavelength peaks around 800 nm, where the absorption and scattering of tissues are minimal. They are coated with a new ligand, which yields small, stable, and bright individual probes in the live cell cytoplasm, even 48 h after the labeling. Furthermore, these near-infrared-emitting quantum dots have a long fluorescence lifetime component (around 150 ns) compared to autofluorescence (
- Subjects :
- MESH: Muscles
Fluorescence-lifetime imaging microscopy
Materials science
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
Cytological Techniques
Biomedical Engineering
Fluorescence in the life sciences
MESH: Spectroscopy, Near-Infrared
Biomaterials
Optics
Quantum Dots
Animals
Humans
MESH: Animals
MESH: Cytological Techniques
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Absorption (electromagnetic radiation)
Spectroscopy
MESH: Optical Imaging
[PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics]
Spectroscopy, Near-Infrared
MESH: Humans
business.industry
Muscles
Optical Imaging
MESH: Quantum Dots
Fluorescence
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
MESH: Cattle
Autofluorescence
Quantum dot
MESH: HeLa Cells
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic
Optoelectronics
Cattle
business
HeLa Cells
Visible spectrum
Subjects
Details
- ISSN :
- 10833668
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....f7c7702ff047d75634a42b1ebbadb395