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Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution
- Source :
- PMC
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Mammalian DNA methylation plays an essential role in development. To date, only snapshots of different mouse and human cell types have been generated, providing a static view on DNA methylation. To enable monitoring of methylation status as it changes over time, we establish a reporter of genomic methylation (RGM) that relies on a minimal imprinted gene promoter driving a fluorescent protein. We show that insertion of RGM proximal to promoter-associated CpG islands reports the gain or loss of DNA methylation. We further utilized RGM to report endogenous methylation dynamics of non-coding regulatory elements, such as the pluripotency-specific super enhancers of Sox2 and miR290. Loci-specific DNA methylation changes and their correlation with transcription were visualized during cell-state transition following differentiation of mouse embryonic stem cells and during reprogramming of somatic cells to pluripotency. RGM will allow the investigation of dynamic methylation changes during development and disease at single-cell resolution.<br />National Institutes of Health (U.S.) (Grant HD 045022)
- Subjects :
- Biology
Article
General Biochemistry, Genetics and Molecular Biology
Mice
03 medical and health sciences
0302 clinical medicine
Epigenetics of physical exercise
Animals
Humans
Promoter Regions, Genetic
DNA Modification Methylases
RNA-Directed DNA Methylation
Embryonic Stem Cells
030304 developmental biology
Epigenomics
0303 health sciences
Biochemistry, Genetics and Molecular Biology(all)
SOXB1 Transcription Factors
Methylation
DNA Methylation
Molecular biology
Cell biology
MicroRNAs
Enhancer Elements, Genetic
DNA methylation
Illumina Methylation Assay
CpG Islands
Single-Cell Analysis
Genomic imprinting
Reprogramming
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 00928674
- Volume :
- 163
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....f7bacbb4a99a2d029351c53ee46c2c82