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A complex choreography of cell movements shapes the vertebrate eye
- Source :
- Development (Cambridge, England)
- Publication Year :
- 2012
- Publisher :
- Company of Biologists, 2012.
-
Abstract
- Optic cup morphogenesis (OCM) generates the basic structure of the vertebrate eye. Although it is commonly depicted as a series of epithelial sheet folding events, this does not represent an empirically supported model. Here, we combine four-dimensional imaging with custom cell tracking software and photoactivatable fluorophore labeling to determine the cellular dynamics underlying OCM in zebrafish. Although cell division contributes to growth, we find it dispensable for eye formation. OCM depends instead on a complex set of cell movements coordinated between the prospective neural retina, retinal pigmented epithelium (RPE) and lens. Optic vesicle evagination persists for longer than expected; cells move in a pinwheel pattern during optic vesicle elongation and retinal precursors involute around the rim of the invaginating optic cup. We identify unanticipated movements, particularly of central and peripheral retina, RPE and lens. From cell tracking data, we generate retina, RPE and lens subdomain fate maps, which reveal novel adjacencies that might determine corresponding developmental signaling events. Finally, we find that similar movements also occur during chick eye morphogenesis, suggesting that the underlying choreography is conserved among vertebrates.
- Subjects :
- Fate map
Retinal pigmented epithelium (RPE)
Time Factors
genetic structures
Chick Embryo
Retinal Pigment Epithelium
Optic cup (anatomical)
Biology
Eye
Retina
03 medical and health sciences
Lens
0302 clinical medicine
Imaging, Three-Dimensional
Fate mapping
Cell Movement
Lens, Crystalline
medicine
Optic vesicle elongation
Morphogenesis
Image Processing, Computer-Assisted
Animals
Molecular Biology
Research Articles
Zebrafish
030304 developmental biology
0303 health sciences
Analysis of Variance
Retinal pigment epithelium
Eye morphogenesis
Cell Cycle
Optic vesicle
Anatomy
eye diseases
medicine.anatomical_structure
Cell tracking
Lens (anatomy)
sense organs
Neuroscience
030217 neurology & neurosurgery
Developmental Biology
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 14779129 and 09501991
- Volume :
- 139
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Development (Cambridge, England)
- Accession number :
- edsair.doi.dedup.....f7ba67039cfbec332674f9d2cf62e698