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Intranasal administration of mesenchymoangioblast-derived mesenchymal stem cells abrogates airway fibrosis and airway hyperresponsiveness associated with chronic allergic airways disease
- Source :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 31(9)
- Publication Year :
- 2017
-
Abstract
- Structural changes known as airway remodeling (AWR) characterize chronic/severe asthma and contribute to lung dysfunction. Thus, we assessed the in vivo efficacy of induced pluripotent stem cell and mesenchymoangioblast-derived mesenchymal stem cells (MCA-MSCs) on AWR in a murine model of chronic allergic airways disease (AAD)/asthma. Female Balb/c mice were subjected to a 9-wk model of ovalbumin (Ova)-induced chronic AAD and treated intravenously or intranasally with MCA-MSCs from weeks 9 to 11. Changes in airway inflammation (AI), AWR, and airway hyperresponsiveness (AHR) were assessed. Ova-injured mice presented with AI, goblet cell metaplasia, epithelial thickening, increased airway TGF-β1 levels, subepithelial myofibroblast and collagen accumulation, total lung collagen concentration, and AHR (all P < 0.001 vs. uninjured control group). Apart from epithelial thickness, all other parameters measured were significantly, although not totally, decreased by intravenous delivery of MCA-MSCs to Ova-injured mice. In comparison, intranasal delivery of MCA-MSCs to Ova-injured mice significantly decreased all parameters measured (all P < 0.05 vs. Ova group) and, most notably, normalized aberrant airway TGF-β1 levels, airway/lung fibrosis, and AHR to values measured in uninjured animals. MCA-MSCs also increased collagen-degrading gelatinase levels. Hence, direct delivery of MCA-MSCs offers great therapeutic benefit for the AWR and AHR associated with chronic AAD.-Royce, S. G., Rele, S., Broughton, B. R. S., Kelly, K., Samuel, C. S. Intranasal administration of mesenchymoangioblast-derived mesenchymal stem cells abrogates airway fibrosis and airway hyperresponsiveness associated with chronic allergic airways disease.
- Subjects :
- 0301 basic medicine
Ovalbumin
Pulmonary Fibrosis
Biochemistry
03 medical and health sciences
Mice
0302 clinical medicine
Fibrosis
Genetics
medicine
Hypersensitivity
Respiratory Hypersensitivity
Animals
Molecular Biology
Administration, Intranasal
Asthma
Goblet cell
Metaplasia
Mice, Inbred BALB C
Lung
biology
business.industry
Mesenchymal stem cell
Mesenchymal Stem Cells
respiratory system
medicine.disease
respiratory tract diseases
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Immunology
biology.protein
Airway Remodeling
Female
Goblet Cells
Airway
business
Myofibroblast
Biotechnology
Stem Cell Transplantation
Subjects
Details
- ISSN :
- 15306860
- Volume :
- 31
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology
- Accession number :
- edsair.doi.dedup.....f7ae5631e26880d5b77f8b59709983cb