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Corticosteroids and montelukast: effects on airway epithelial and human umbilical vein endothelial cells
- Source :
- Lung. 188(3)
- Publication Year :
- 2009
-
Abstract
- Our primary objective was to investigate the possible contribution of controller medications to asthmatic airway remodeling, by (1) comparing the apoptotic and necrotic effects of several corticosteroids and montelukast on cultured airway human bronchial surface epithelial (16HBE) and submucosal (Calu3) cells; (2) measuring epithelial shedding potential and desmosome length in response to a cytokine challenge, with or without co-administered corticosteroids; and (3) studying corticosteroids and montelukast effects on inter-cellular adhesion molecule (ICAM) expression in both 16HBE and human umbilical vein endothelial cells (HUVEC). For this purpose, apoptosis, necrosis, and ICAM expression were quantified by flow cytometry, with 16HBE cells sensitive to both the apoptotic and necrotic effects of dexamethasone and montelukast; Calu3 cells sensitive only to budesonide. Transmission electron microscopy revealed decreased desmosome length in the presence of cytokines (TNF-alpha and INF-gamma), with corticosteroids counteracting this reduction. Dexamethasone, beclomethasone, and montelukast decreased versus increased ICAM-1 expression in airway epithelial cells and HUVEC, respectively. For conclusions, bronchial surface epithelial and submucosal cells exhibit a different sensitivity profile toward dexamethasone, budesonide, and montelukast, with corticosteroids preventing cytokineinduced desmosomal damage in 16HBE cells. The studied drugs led to increased ICAM-1 expression in endothelium, potentially facilitating inflammatory cell migration into lung tissue.
- Subjects :
- Pulmonary and Respiratory Medicine
Cyclopropanes
Pathology
medicine.medical_specialty
Umbilical Veins
Necrosis
Endothelium
Apoptosis
macromolecular substances
Respiratory Mucosa
Acetates
Sulfides
Umbilical vein
Cell Line
Adrenal Cortex Hormones
medicine
Humans
Anti-Asthmatic Agents
Montelukast
business.industry
Desmosomes
Intercellular Adhesion Molecule-1
Asthma
medicine.anatomical_structure
Asthmatic Airway Remodeling
Immunology
Quinolines
Respiratory epithelium
Cytokines
Leukotriene Antagonists
Endothelium, Vascular
medicine.symptom
Airway
business
medicine.drug
Subjects
Details
- ISSN :
- 14321750
- Volume :
- 188
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Lung
- Accession number :
- edsair.doi.dedup.....f785452677cffccfef80f78cdf6dd47e