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Molecular cloning of Y-Box binding protein-1 from mandarin fish and its roles in stress-response and antiviral immunity
- Source :
- Fish & Shellfish Immunology. 93:406-415
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Mandarin fish (Siniperca chuatsi) is a universally farmed fish species in China and has a large farming scale and economic value. With the high-density cultural mode in mandarin fish, viral diseases, such as infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdovirus (SCRV), have increased loss, which has seriously restricted the development of aquaculture. Y-Box binding protein 1 (YB-1) is a member of cold shock protein family that regulates multiple cellular processes. The roles of mammalian YB-1 protein in environmental stress and innate immunity have been studied well, but its roles in teleost fishes remain unknown. In the present study, the characteristic of S. chuatsi YB-1 (scYB-1) and its roles in cold stress and virus infection were investigated. The scYB-1 obtained an 1541 bp cDNA that contains a 903 bp open reading frame encoding a protein of 300 amino acids. Tissue distribution results showed that the scYB-1 is a ubiquitously expressed gene found among tissues from mandarin fish. Overexpression of scYB-1 can increase the expression levels of cold shock-responsive genes, such as scHsc70a, scHsc70b, and scp53. Furthermore, the role of scYB-1 in innate immunity was also investigated in mandarin fish fry (MFF-1) cells. The expression level of scYB-1 was significant change in response to poly (I:C), poly (dG:dC), PMA, ISKNV, or SCRV stimulation. The overexpression of scYB-1 can significantly increase the expression levels of NF-κB-responsive genes, including scIL-8, scTNF-α, and scIFN-h. The NF-κB-luciferase report assay results showed that the relative expression of luciferin was significantly increased in the cells overexpressed with scYB-1 compared with those in cells overexpressed with control plasmid. These results indicate that scYB-1 can induce the NF-κB signaling pathway in MFF-1 cells. Overexpressed scYB-1 can downregulate the expression of ISKNV viral major capsid protein (mcp) gene but upregulates the expression of SCRV mcp gene. Moreover, knockdown of scYB-1 using siRNA can upregulate the expression of ISKNV mcp gene but downregulates the expression of SCRV mcp gene. These results indicate that scYB-1 suppresses ISKNV infection while enhancing SCRV infection. The above observations suggest that scYB-1 is involved in cold stress and virus infection. Our study will provide an insight into the roles of teleost fish YB-1 protein in stress response and innate immunity.
- Subjects :
- Fish Proteins
0301 basic medicine
Aquatic Science
Biology
Fish Diseases
03 medical and health sciences
Polydeoxyribonucleotides
Rhabdoviridae Infections
Complementary DNA
Gene expression
Animals
Environmental Chemistry
Amino Acid Sequence
Gene
Phylogeny
Gene knockdown
Innate immune system
Base Sequence
Gene Expression Profiling
Binding protein
Fishes
04 agricultural and veterinary sciences
General Medicine
Y box binding protein 1
DNA Virus Infections
Immunity, Innate
Iridoviridae
Cell biology
Poly I-C
030104 developmental biology
Gene Expression Regulation
040102 fisheries
Tetradecanoylphorbol Acetate
0401 agriculture, forestry, and fisheries
Y-Box-Binding Protein 1
Rhabdoviridae
Sequence Alignment
Kidney necrosis
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....f775e6681b8042fc2e743608a92d1161