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EC-18, a synthetic monoacetyldiglyceride (1-palmitoyl-2-linoleoyl-3-acetylglycerol), attenuates the asthmatic response in an aluminum hydroxide/ovalbumin-induced model of asthma
- Source :
- International Immunopharmacology. 18:116-123
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- EC-18 is a synthetic monoacetyldiaglyceride that is a major constituent in antlers of Sika deer (Cervus nippon Temmenick). In this study, we evaluated the protective effects of EC-18 on Th2-type cytokines, eosinophil infiltration, and other factors in an aluminum hydroxide/ovalbumin (OVA)-induced murine asthma model. Mice were sensitized on days 0 and 14 by intraperitoneal injection of OVA with aluminum hydroxide. On days 21, 22 and 23 after the initial sensitization, the mice received an airway challenge with OVA for 1h using an ultrasonic nebulizer. EC-18 was administered to mice by oral gavage at doses of 30mg/kg and 60mg/kg once daily from day 18 to 23. Methacholine responsiveness was measured 24h after the final OVA challenge, and the bronchoalveolar lavage fluid (BALF) was collected 48h after the final OVA challenge. EC-18 significantly reduced methacholine responsiveness, T helper type 2 (Th2) cytokines, eotaxin-1, immunoglobulin (Ig) E, IgG, and the number of inflammatory cells. In addition, EC-18-treated mice exhibited the reduction in the expression of inducible nitric oxide synthase (iNOS) in lung tissue. In the histological analysis using hematoxylin-eosin stain and periodic acid-Schiff stain, EC-18 attenuated the infiltration of inflammatory cells into the airway and reduced the level of mucus production. Our results showed that EC-18 effectively suppressed the asthmatic response induced by OVA challenge. These effects were considered to be associated with iNOS suppression. In conclusion, this study suggests that EC-18 may be a therapeutic agent for allergic asthma.
- Subjects :
- Ovalbumin
medicine.medical_treatment
Immunology
Intraperitoneal injection
Nitric Oxide Synthase Type II
Aluminum Hydroxide
Pharmacology
Bronchial Provocation Tests
Diglycerides
Mice
Th2 Cells
Cell Movement
medicine
Animals
Humans
Immunology and Allergy
Anti-Asthmatic Agents
Lung
Cells, Cultured
Sensitization
Mice, Inbred BALB C
medicine.diagnostic_test
biology
Chemistry
respiratory system
Eosinophil
Asthma
Eosinophils
Nitric oxide synthase
Disease Models, Animal
Bronchoalveolar lavage
medicine.anatomical_structure
biology.protein
Airway Remodeling
Cytokines
Female
Methacholine
Antibody
medicine.drug
Subjects
Details
- ISSN :
- 15675769
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- International Immunopharmacology
- Accession number :
- edsair.doi.dedup.....f768e9dc8b327651dcb56c9b4a0067bc