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Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning
- Source :
- Genome Research. 9:1277-1287
- Publication Year :
- 1999
- Publisher :
- Cold Spring Harbor Laboratory, 1999.
-
Abstract
- Double anal fin (Da) is a medaka with an autosomal semidominant mutation that causes mirror image duplication of the ventral region concentrating on the caudal region. The chromosomal location of the Da gene and its sequence have remained unknown. We constructed a medaka linkage map as a first step to approach positional cloning of the gene. The segregation analysis was performed on the basis of genetic recombination during female meiosis using 134 random amplified polymorphic DNA (RAPD) markers, 13 sequence-tagged sites (STSs), 15 polymorphic sequences from known genes, and the Da gene. One hundred forty-six markers from the above markers segregated into 26 linkage groups. The size of the genome was estimated to be 1776 cM in length. We identified four syntenic regions between medaka and zebrafish (and human) by mapping the known genes and found one of them to be located in close proximity to theDa gene. By mapping the region surrounding the Dagene in high resolution, two markers were detected flanking theDa gene at 0.32 and 0.80 cM. The detected markers providing a vital clue to initiate chromosome walking will lead us to the definite location of the Da gene.
- Subjects :
- Genetic Markers
Letter
Positional cloning
Oryzias
Locus (genetics)
Biology
Genetic recombination
Gene mapping
Genetic linkage
Sequence Homology, Nucleic Acid
Gene duplication
Genetics
Primer walking
Animals
Humans
Cloning, Molecular
Gene
Zebrafish
Genetics (clinical)
Body Patterning
Genes, Dominant
Sequence Tagged Sites
Polymorphism, Genetic
Gene Amplification
Chromosome Mapping
Meiosis
Phenotype
Mutation
Female
Subjects
Details
- ISSN :
- 15495469 and 10889051
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Genome Research
- Accession number :
- edsair.doi.dedup.....f76341e079eb9e6a26d3d663cf8c2168
- Full Text :
- https://doi.org/10.1101/gr.9.12.1277