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Effect of IVIG Formulation on IgG Binding to Self- and Exo- Antigens In Vitro and In Vivo
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 8, p e0161826 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- In relation to the recent trials of Intravenous Immunoglobulin (IVIG) in Alzheimer's Disease (AD) it was demonstrated that different IgG preparations contain varying amounts of natural anti-amyloid β (Aβ) antibodies as measured by ELISA. We therefore investigated the relevance of ELISA data for measuring low-affinity antibodies, such as anti-Aβ. We analysed the binding of different commercial Immunoglobulin G (IgG) preparations to Aβ, actin and tetanus toxoid in different binding assays to further investigate the possible cause for observed differences in binding to Aβ and actin between different IgG preparations. We show that the differences of commercial IgG preparations in binding to Aβ and actin in ELISA assays are artefactual and only evident in in vitro binding assays. In functional assays and in vivo animal studies the different IVIG preparations exhibited very similar potency. ELISA data alone are not appropriate to analyse and rank the binding capacity of low-affinity antibodies to Aβ or other endogenous self-antigens contained in IgG preparations. Additional analytical methods should be adopted to complement ELISA data.
- Subjects :
- 0301 basic medicine
Bacterial Diseases
Herpesvirus 3, Human
Physiology
Antibody Affinity
lcsh:Medicine
Toxicology
Pathology and Laboratory Medicine
Biochemistry
Autoantigens
Immunoglobulin G
Binding Analysis
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
Tetanus Toxoid
Toxins
Enzyme-Linked Immunoassays
Amino Acids
lcsh:Science
Multidisciplinary
Immune System Proteins
biology
Chemistry
Organic Compounds
Toxoid
Immunoglobulins, Intravenous
Hematology
Toxoids
Body Fluids
Infectious Diseases
Blood
IgG binding
Physical Sciences
Female
Antibody
Anatomy
Research Article
Protein Binding
Proline
Immunology
Toxic Agents
Bacterial Toxins
Glycine
Enzyme-Linked Immunosorbent Assay
Research and Analysis Methods
Antibodies
Blood Plasma
03 medical and health sciences
Antigen
In vivo
Alzheimer Disease
Potency
Animals
Humans
Antigens
Immunoassays
Chemical Characterization
Tetanus
Amyloid beta-Peptides
lcsh:R
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
Proteins
Cyclic Amino Acids
Molecular biology
In vitro
Actins
Peptide Fragments
Rats
030104 developmental biology
Aliphatic Amino Acids
biology.protein
Immunologic Techniques
lcsh:Q
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....f735247a2ec7dae3640ab0b5f4f71ed5