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Organ culture study of effect of vitamin-A-deficiency on rat third molar development
- Source :
- Archives of oral biology. 29(11)
- Publication Year :
- 1984
-
Abstract
- A culture procedure for rat third molars suitable for nutritional-developmental studies is described. Urerupted third molars from 12-day-old rats were cultured in BGJ b media containing 20 per cent rat serum and supplemented with 25 mM HEPES buffer, 25 mg ascorbic acid, 20 mg L-glutamine, 12mg penicillin G and 10mg streptomycin sulphate per 100 ml of media. Molars were cultured at the liquid-gas interphase using a 50 per cent O 2 , 45 per cent N 2 , 5 per cent CO 2 gas mixture at 10 lb-psig (pounds per square inch guage). Molar cultures were maintained successfully for 9–14 days without evidence of necrosis, although they developed at a slower rate than in vivo . Molars cultured in 50 per cent O 2 compared to those cultured in 21 per cent O 2 for periods of 2, 4, 6 and 8 days had higher values for protein, alkaline phosphatase (AP), Ca, P and Ca/P. Vitamin- A -deficiency gave lower values for AP, Ca, P, Ca/P, 45 Ca, 35 S and [ 14 C]-proline uptake. Histologically, A -molars had atrophie ameloblasts, some foci of squamous metaplasia and abnormal keratin formation. Thus, deficiency of vitamin A imposed during in-vitro development of rat third molars retarded dentinogenesis and interfered with early mineralization of enamel and dentine.
- Subjects :
- Molar
Vitamin
Aging
Organ culture
Andrology
chemistry.chemical_compound
Organ Culture Techniques
Oxygen Consumption
stomatognathic system
medicine
Animals
Pounds per square inch
General Dentistry
Chemistry
Vitamin A Deficiency
Rats, Inbred Strains
Cell Biology
General Medicine
Anatomy
Dentinogenesis
Ascorbic acid
medicine.disease
Squamous metaplasia
Culture Media
Rats
Vitamin A deficiency
Otorhinolaryngology
Alkaline phosphatase
Odontogenesis
Tooth Calcification
Subjects
Details
- ISSN :
- 00039969
- Volume :
- 29
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Archives of oral biology
- Accession number :
- edsair.doi.dedup.....f6dc6733ee602bc20d5f9b5e7d446730