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Progression and regression of myointimal hyperplasia in experimental vein grafts depends on platelet-derived growth factor and basic fibroblastic growth factor production
- Source :
- Journal of Vascular Surgery. (4):568-575
- Publisher :
- Published by Mosby, Inc.
-
Abstract
- Purpose: The factors that lead to myointimal hyperplasia (MH) in arterial vein grafts (AVGs) are unknown. Platelet-derived growth factor (PDGF) and basic fibroblastic growth factor (bFGF) are two powerful mitogens for smooth muscle cells that have been implicated in the genesis of MH. The aim of this study was to analyze the correlation between progression and regression of MH and production of PDGF and bFGF in experimental vein grafts.Materials: In 64 inbred Lewis rats, a 1-cm segment of inferior vena cava was inserted at the level of the abdominal aorta. The segments of inferior vena cava were obtained from syngenic rats. In 48 rats, the AVG was explanted 3 days (n=8), 7 days (n=8), 4 weeks (n=24), and 12 weeks (n=8) after surgery. In 16 rats the vein graft was explanted after being in the arterial system for 4 weeks and was reimplanted as a venous-venous bypass in syngenic Lewis rats. Reimplanted vein grafts (RVGs) were explanted 2 weeks (n=8) and 8 weeks (n=8) later. Grafts were analyzed by light and electron microscopy, morphometry, and histochemistry, and were put in organ culture to assess PDGF and bFGF production and mitogenic activity.Results: We observed MH formation in AVGs and MH regression in RVGs (p
- Subjects :
- Male
Pathology
Platelet-derived growth factor
Vena Cava
medicine.medical_treatment
Remission, Spontaneous
Muscle, Smooth, Vascular
Mice
chemistry.chemical_compound
Conditioned
Aorta, Abdominal
Aorta
Platelet-Derived Growth Factor
Microscopy
3T3 Cells
Animals
Culture Media, Conditioned
Disease Progression
Fibroblast Growth Factor 2
Histocytochemistry
Hyperplasia
Microscopy, Electron
Mitogens
Organ Culture Techniques
Rats
Rats, Inbred Lew
Replantation
Tunica Intima
Veins
Vena Cava, Inferior
Inbred Lew
biology
Abdominal aorta
medicine.vein
cardiovascular system
Muscle
Smooth
Cardiology and Cardiovascular Medicine
Platelet-derived growth factor receptor
Inferior
medicine.medical_specialty
Remission
Organ culture
Electron
Inferior vena cava
Vascular
medicine.artery
medicine
Abdominal
business.industry
Spontaneous
Growth factor
medicine.disease
Culture Media
chemistry
biology.protein
Surgery
business
Subjects
Details
- Language :
- English
- ISSN :
- 07415214
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of Vascular Surgery
- Accession number :
- edsair.doi.dedup.....f6d2288e6608166d22535fb3de35f56f
- Full Text :
- https://doi.org/10.1016/S0741-5214(96)80034-6