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The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation
- Source :
- Biochemical and Biophysical Research Communications. 513:159-165
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The magnesium transporters TRPM7 and MagT1 are overexpressed in osteoblastogenesis. We have shown that silencing either TRPM7 or MagT1 accelerates the osteogenic differentiation of human bone mesenchymal stem cells. Here we demonstrate that the simultaneous downregulation of TRPM7 and MagT1 inhibits cell growth and activates autophagy, which is required in the early phases of osteoblastogenesis. In TRPM7/MagT1 downregulating cells the expression of two transcription factors required for activating osteogenesis, i.e. RUNX2 and OSTERIX, is induced more than in the controls both in the presence and in the absence of osteogenic stimuli, while COL1A1 is upregulated in co-silencing cells as much as in the controls. This explains why we found no differences in calcium deposition. We conclude that one of the two transporters should be expressed to accelerate osteogenic differentiation.
- Subjects :
- Adult
0301 basic medicine
Biophysics
Down-Regulation
TRPM Cation Channels
Protein Serine-Threonine Kinases
Biochemistry
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Osteogenesis
TRPM7
Autophagy
Humans
Gene silencing
Cation Transport Proteins
Molecular Biology
Transcription factor
Cells, Cultured
Cell Proliferation
Chemistry
Cell growth
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Cell biology
RUNX2
030104 developmental biology
030220 oncology & carcinogenesis
RNA Interference
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 513
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....f6c7ef645e66445b07325728e27b5d0e