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Identification and characterization of novel small RNAs in the aspS–yrvM intergenic region of the Bacillus subtilis genome
- Source :
- Microbiology. 148:2591-2598
- Publication Year :
- 2002
- Publisher :
- Microbiology Society, 2002.
-
Abstract
- A novel RNA species was isolated from Bacillus subtilis, and its sequence was determined and mapped to its genetic position. This RNA was termed BS190 RNA from the length of its mature form (190 nt), and the gene encoding it is located within the aspS-yrvM intergenic region of the B. subtilis genome. Northern blotting revealed that the novel RNA species is transcribed in vegetative cells as a larger precursor (BS201 RNA, 201 nt). After transcription, the 5' end of the precursor is processed to generate the mature form, BS190 RNA. A computer-aided prediction of the secondary structure of BS190 RNA showed that it can be folded into a single hairpin structure with some bulge structures. The authors found that the growth rate of a DeltaBS190 mutant strain of B. subtilis was reduced when compared to the wild-type. A phylogenetic comparison of the sequence of the BS190 RNA gene with sequences from the databases suggests that RNA related to BS190 RNA appears to be encoded in the genomes of Bacillus halodurans and Listeria monocytogenes.
- Subjects :
- DNA, Bacterial
Genetics
RNA, Untranslated
Base Sequence
Transcription, Genetic
Molecular Sequence Data
5.8S ribosomal RNA
Intron
Chromosome Mapping
RNA
RNA-dependent RNA polymerase
Sequence Analysis, DNA
Biology
Non-coding RNA
Microbiology
Molecular biology
RNA, Bacterial
RNA silencing
RNA editing
Nucleic Acid Conformation
DNA, Intergenic
RNA Processing, Post-Transcriptional
Genome, Bacterial
Small nuclear RNA
Bacillus subtilis
Subjects
Details
- ISSN :
- 14652080 and 13500872
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- Microbiology
- Accession number :
- edsair.doi.dedup.....f6804757ce840827adb52a815c681fb5