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Expression pattern of two paralogs of the PI/GLO-like locus during Orchis italica (Orchidaceae, Orchidinae) flower development
- Source :
- Development Genes and Evolution. 221:241-246
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- The class B MADS-box genes belong to two distinct functional groups: the AP3/DEF-like and the PI/GLO-like sub-families. In orchids, AP3/DEF-like genes are present in four copies, each with a different role in floral organ formation, which is described in the "orchid code" model. Interestingly, the orchid PI/GLO-like genes are present in two copies in Orchidinae, whereas they are described as single copy in the other orchid lineages. The two PI/GLO-like paralogs have site-specific different selective constraints; in addition, they show relaxation of purifying selection when compared to the single-copy lineages. In this study, we present a comparative analysis of the expression patterns of the two PI/GLO-like paralogs, OrcPI and OrcPI2, in floral tissues of Orchis italica in different developmental stages using real-time PCR. The two genes show similar expression profiles in the tissue examined, with differences detectable between immature and mature inflorescence. In all cases, OrcPI2 is expressed at a higher level than OrcPI. Real-time PCR results reveal that the co-expression of the two duplicated loci could have a fully or partially redundant function. The possible evolutionary fate of OrcPI and OrcPI2 is discussed as well as their involvement in ovary development.
- Subjects :
- Homeodomain Proteins
Genetics
Orchidaceae
Gene duplication
biology
MADS-box genes
Locus (genetics)
Flowers
biology.organism_classification
Orchidinae
Floral organ formation
Negative selection
Inflorescence
Gene Expression Regulation, Plant
Orchis italica
Expression pattern
Gene
Plant Proteins
Developmental Biology
Subjects
Details
- ISSN :
- 1432041X and 0949944X
- Volume :
- 221
- Database :
- OpenAIRE
- Journal :
- Development Genes and Evolution
- Accession number :
- edsair.doi.dedup.....f66ccf9e9eedb7a1aee57939c46ec718