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CO ameliorates endothelial senescence induced by 5-fluorouracil through SIRT1 activation
- Source :
- Archives of biochemistry and biophysics. 677
- Publication Year :
- 2019
-
Abstract
- Endothelial senescence is the main risk factor that contributes to vascular dysfunction and the progression of vascular disease. Carbon monoxide (CO) plays an important role in preventing vascular dysfunction and in maintaining vascular physiology or homeostasis. The application of exogenous CO has been shown to confer protection in several models of cardiovascular injury or disease, including hypertension, atherosclerosis, balloon-catheter injury, and graft rejection. However, the mechanism by which CO prevents endothelial senescence has been largely unexplored. The aim of this study was to evaluate the effects of CO on endothelial senescence and to investigate the possible mechanisms underlying this process. We measured the levels of senescence-associated-β-galactosidase activity, senescence-associated secretory phenotype, reactive oxygen species (ROS) production, and stress granule in human umbilical vein endothelial cells and the WI-38 human diploid fibroblast cell line. We found that 5-fluorouracil (5FU)-induced ROS generation was inhibited by CO-releasing molecules (CORM)-A1 treatment, and endothelial senescence induced by 5FU was attenuated by CORM-A1 treatment. The SIRT1 inhibitor EX527 reversed the inhibitory effect of CO on the 5FU-induced endothelial senescence. Furthermore, SIRT1 deficiency abolished the stress granule formation by CO. Our results suggest that CO alleviates the endothelial senescence induced by 5FU through SIRT1 activation and may hence have therapeutic potential for the treatment of vascular diseases.
- Subjects :
- 0301 basic medicine
Nitric Oxide Synthase Type III
Biophysics
Down-Regulation
Biochemistry
Umbilical vein
Antioxidants
03 medical and health sciences
Stress granule
Sirtuin 1
medicine
Human Umbilical Vein Endothelial Cells
Humans
Molecular Biology
Cellular Senescence
chemistry.chemical_classification
Reactive oxygen species
Carbon Monoxide
030102 biochemistry & molecular biology
Chemistry
Vascular disease
medicine.disease
Phenotype
Cell biology
030104 developmental biology
Fluorouracil
Cardiovascular Injury
Reactive Oxygen Species
Homeostasis
Heme Oxygenase-1
medicine.drug
Subjects
Details
- ISSN :
- 10960384
- Volume :
- 677
- Database :
- OpenAIRE
- Journal :
- Archives of biochemistry and biophysics
- Accession number :
- edsair.doi.dedup.....f6679adbda2cae898205b71a448256ff