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Shear stress induces endothelial transdifferentiation from mouse smooth muscle cells
- Source :
- Biochemical and Biophysical Research Communications. 346:860-865
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Smooth muscle cells (SMCs) under shear stress may alter their gene expression patterns to adapt to a new hemodynamic environment. Their plasticity may play an important role in vascular development, healing, and remodeling as well as vascular lesion formation under abnormal environmental conditions. A mouse vascular SMC line (P53LMACO1) cultured under shear stress significantly increased the mRNA levels of endothelial cell markers including Platelet-endothelial cell adhesion molecule-1 (PECAM-1), von Willebrand factor (vWF), and VE-cadherin, while significantly decreasing the mRNA levels of SMC markers including alpha-smooth muscle actin (alpha-SMA), calponin-1, smooth muscle myosin heavy chain (SMMHC), and transgelin as compared to static control cells. Protein levels of PECAM-1 and vWF were significantly increased, while protein levels of alpha-SMA were substantially decreased in the shear stress-cultured cells. In addition, shear stress-cultured cells showed an enhanced capability to form capillary-like structures on Matrigel. Thus, shear stress may promote endothelial cell transdifferentiation from SMCs.
- Subjects :
- Cellular differentiation
Myocytes, Smooth Muscle
Biophysics
Biology
Biochemistry
Cell Line
Mice
Antigens, CD
von Willebrand Factor
Animals
Myocyte
RNA, Messenger
Cell adhesion
Cell Shape
Molecular Biology
Matrigel
Transdifferentiation
Endothelial Cells
Cell Differentiation
Cell Biology
Cadherins
Cell biology
Platelet Endothelial Cell Adhesion Molecule-1
Endothelial stem cell
Calponin 1
Gene Expression Regulation
Cell culture
cardiovascular system
Biomarkers
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 346
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....f64be2d4c31e5817956ea10e1370bbdf