Microbial explanation to performance stratification along up-flow solid-phase denitrification column packed with polycaprolactone

In this study, the fluctuating profiles of physicochemical and microbial characterizations along different filling heights of continuously up-flow solid-phase denitrification (SPD) columns packed with polycaprolactone (PCL) were investigated. It was found both the PCL filling area and non-filling area made significant contributions to treatment performance and denitrification mainly occurred near the bottom of the filling column. Nitrate displayed a high proportional removal (≥98.7%) among all the cases except the one with the lowest filling ratio (FR30) and highest NLR (3.99 ± 0.12 gN/(L·d)), while nitrite and ammonium displayed a weak accumulation in final effluents (nitrite ≤ 0.40 mg/L; ammonium ≤ 0.98 mg/L). The intensity of PCL hydrolysis in the top substrate was stronger than those in the middle or bottom. Both dissimilatory nitrate reduction to ammonium (DNRA) and microbial lysis contributed to ammonium accumulation, and nitrate was mainly removed via traditional denitrification and DNRA. JGI_0000069-P22_unclassified and Gracilibacteria_unclassified might contribute to denitrification.