Investigating the role of dissolved and droplet oil in aquatic toxicity using dispersed and passive dosing systems

Characterization of the aquatic toxicity of oil is needed to support hazard assessment and inform spill response. Natural processes and mitigation strategies involving dispersant use can result in exposures to both dissolved and droplet oil that are not typically differentiated when oil exposures are characterized in toxicity tests. Thus, the impact of droplets on aquatic toxicity is largely uncharacterized. To improve the understanding of the role of droplets, acute toxicity tests with Daphnia magna and Americamysis bahia were performed with Endicott crude oil in low-energy mixing systems with and without Corexit 9500 dispersant. Exposures were also prepared by placing crude oil in silicone tubing and passively dosing test media to provide dissolved oil exposures without droplets. A framework is described for characterizing dissolved phase exposures using both mechanistic modeling and passive sampling measurements. The approach is then illustrated by application to data from the present study. Expression of toxicity in terms of toxic units calculated from modeled dissolved oil concentrations or passive sampling measurements showed similar dose responses between exposure systems and organisms, despite the gradient in droplet oil. These results indicate that droplets do not appreciably contribute to toxicity for the 2 species investigated and further support hazard evaluation of dispersed oil on the basis of dissolved exposure metrics. Environ Toxicol Chem 2017;36:1020-1028. © 2016 SETAC.