Back to Search
Start Over
Fluorescent Protein Expressing Rickettsia buchneri and Rickettsia peacockii for Tracking Symbiont-Tick Cell Interactions
- Source :
- Veterinary Sciences; Volume 3; Issue 4; Pages: 34, Veterinary Sciences, Vol 3, Iss 4, p 34 (2016), Veterinary Sciences
- Publication Year :
- 2016
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2016.
-
Abstract
- Rickettsiae of indeterminate pathogenicity are widely associated with ticks. The presence of these endosymbionts can confound a One Health approach to combatting tick-borne diseases. Genomic analyses of symbiotic rickettsiae have revealed that they harbor mutations in gene coding for proteins involved in rickettsial pathogenicity and motility. We have isolated and characterized two rickettsial symbionts-Rickettsia peacockii and R. buchneri-both from ticks using tick cell cultures. To better track these enigmatic rickettsiae in ticks and at the tick-mammal interface we transformed the rickettsiae to express fluorescent proteins using shuttle vectors based on rickettsial plasmids or a transposition system driving insertional mutagenesis. Fluorescent protein expressing R. buchneri and R. peacockii will enable us to elucidate their interactions with tick and mammalian cells, and track their location and movement within individual cells, vector ticks, and host animals.
- Subjects :
- 0301 basic medicine
green fluorescent protein
spectinomycin
030106 microbiology
Tick
Article
shuttle vector
Himar 1 transposition
Microbiology
Green fluorescent protein
ticks
Insertional mutagenesis
03 medical and health sciences
Plasmid
endosymbionts
Shuttle vector
parasitic diseases
Gene
Rickettsia buchneri
lcsh:Veterinary medicine
General Veterinary
biology
Rickettsia peacockii
transformation
mCherry fluorescent protein
rifampin
biology.organism_classification
bacterial infections and mycoses
Virology
3. Good health
Transformation (genetics)
lcsh:SF600-1100
bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 23067381
- Database :
- OpenAIRE
- Journal :
- Veterinary Sciences; Volume 3; Issue 4; Pages: 34
- Accession number :
- edsair.doi.dedup.....f636e2ec2a870c9cdccbf44a5e414e9a
- Full Text :
- https://doi.org/10.3390/vetsci3040034