Identification of 677C>T Polymorphism of LRP1 Gene by PCR-RFLP Method

The 677 C>T (rs1799986) polymorphism is located at position 677 in exon 3 of the LRP1 gene. The aim of this study was to determine the allele and genotype frequencies of the polymorphism in Bosnian population. The study included 100 unrelated healthy individuals. Genotyping of 677 C>T polymorphism of the LRP1 gene was performed with PCR-RFLP method. The most frequent allele was the C allele (91%), while the T allele was represented by 9%. These results suggested that presence of the 677 C>T polymorphism of the LRP1 gene in our population should be a base for further case-control association or population genetics studies. Keywords –LRP1 gene, PCR-RFLP method.