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Molecular microbiological evaluation of subgingival biofilm sampling by paper point and curette
- Source :
- APMIS. 122:347-352
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- The present clinical study aimed to investigate if there are differences in microbiological outcomes dependent on the subgingival biofilm collection method. Subgingival biofilm samples were collected from the four deepest pockets (>5 mm) of 17 patients with aggressive periodontitis (AgP) and 33 patients with chronic periodontitis (CP), first by paper point and thereafter by curette. Samples obtained with the same method were pooled together from each patient and forwarded for molecular microbiological analysis by a commercially available assay (IAI Pado Test 4.5) that estimates total bacterial load and levels of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Aggregatibacter actinomycetemcomitans. Data analysis included frequency of detection, quantification and correlation of detection levels between the two sampling methods. P. gingivalis, T. forsythia and T. denticola were detected in >90% of the samples, and their detection levels exhibited a strong correlation between sampling methods. The detection consistency of A. actinomycetemcomitans was 56% between the two sampling methods. A. actinomycetemcomitans was more readily detected by paper point compared with curette collection with a stronger correlation between the two methods in AgP. Subgingival biofilm sampling by curette or paper point does not yield differences in the detection of the three 'red complex' species. However, A. actinomycetemcomitans was more consistently detected by means of paper point collection, which can be crucial in the decision to administer antibiotics as an adjunctive periodontal treatment.
- Subjects :
- Male
Paper
Microbiology (medical)
Dentistry
610 Medicine & health
Aggregatibacter actinomycetemcomitans
2726 Microbiology (medical)
Pathology and Forensic Medicine
Microbiology
RNA, Ribosomal, 16S
10066 Clinic of Conservative and Preventive Dentistry
medicine
Humans
Periodontal Pocket
Immunology and Allergy
Tannerella forsythia
Aggressive periodontitis
Porphyromonas gingivalis
Periodontitis
Bacteriological Techniques
biology
Bacteroidetes
Curette
business.industry
Treponema denticola
General Medicine
biology.organism_classification
medicine.disease
Chronic periodontitis
Bacterial Load
10182 Institute of Oral Biology
2734 Pathology and Forensic Medicine
RNA, Bacterial
Aggressive Periodontitis
Molecular Diagnostic Techniques
Biofilms
Chronic Periodontitis
2723 Immunology and Allergy
Female
business
Subjects
Details
- ISSN :
- 09034641
- Volume :
- 122
- Database :
- OpenAIRE
- Journal :
- APMIS
- Accession number :
- edsair.doi.dedup.....f5f5582ebf3488a413d57bfde842cb9e