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Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes

Authors :
Liu Zhuoqi
Jiaqi Yu
Xiaoguo Jiao
Chunxiao Yang
Guo Wei
Huipeng Pan
Lan Kong
Jing Lü
Source :
Horticultural Plant Journal, Vol 7, Iss 2, Pp 142-148 (2021)
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia. Here, we employed transcriptome sequencing of three female and three male H. vigintioctopunctata libraries to identify sex-related genes. We recorded 281 599 294 bp of clean reads, and de novo assembly generated 85 206 unigenes, with N50 of 1 311 bp and average length of 763 bp. We found 5 002 genes highly expressed in males and 2 179 genes highly expressed in females. Expression profiles of six unigenes specific to females, six unigenes specific to males, and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR; results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis. Using all unigenes as references, we identified 6 657 putative simple sequence repeats. The transcriptome sequences and gene expression profiles of female and male H. vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.

Details

ISSN :
24680141
Volume :
7
Database :
OpenAIRE
Journal :
Horticultural Plant Journal
Accession number :
edsair.doi.dedup.....f5d9b0a81842add8697dafc1383453ac
Full Text :
https://doi.org/10.1016/j.hpj.2020.08.003