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Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes
- Source :
- Horticultural Plant Journal, Vol 7, Iss 2, Pp 142-148 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia. Here, we employed transcriptome sequencing of three female and three male H. vigintioctopunctata libraries to identify sex-related genes. We recorded 281 599 294 bp of clean reads, and de novo assembly generated 85 206 unigenes, with N50 of 1 311 bp and average length of 763 bp. We found 5 002 genes highly expressed in males and 2 179 genes highly expressed in females. Expression profiles of six unigenes specific to females, six unigenes specific to males, and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR; results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis. Using all unigenes as references, we identified 6 657 putative simple sequence repeats. The transcriptome sequences and gene expression profiles of female and male H. vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Henosepilachna vigintioctopunctata
Sequence assembly
Plant Science
lcsh:Plant culture
01 natural sciences
Biochemistry, Genetics and Molecular Biology (miscellaneous)
differential expression
Transcriptome
03 medical and health sciences
Gene expression
sex
lcsh:SB1-1110
Gene
Ecology, Evolution, Behavior and Systematics
Genetics
Ecology
biology
Renewable Energy, Sustainability and the Environment
biology.organism_classification
Sexual dimorphism
030104 developmental biology
Microsatellite
PEST analysis
RNA-seq
transcriptome annotation
010606 plant biology & botany
Subjects
Details
- ISSN :
- 24680141
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Horticultural Plant Journal
- Accession number :
- edsair.doi.dedup.....f5d9b0a81842add8697dafc1383453ac
- Full Text :
- https://doi.org/10.1016/j.hpj.2020.08.003