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Patterns of cell death triggered in two different cell lines by HypF-N prefibrillar aggregates
- Publication Year :
- 2005
-
Abstract
- The finding that aggregates of disease-unrelated proteins can induce cell death indicates that proteins may act as potential toxins. Cells experiencing toxic protein aggregates often display modifications of the redox status and ion balance, eventually leading to apoptosis; however, in some cases, tissue and cultured cell types die with features of necrosis. To elucidate the pathways leading to such different outcomes, we studied the biochemical features of death in H-END and NIH/3T3 cells exposed to prefibrillar aggregates of a disease-unrelated protein. The two types of cells died by apoptosis and necrosis, respectively. The pattern of caspase and proapoptotic factor activation was investigated together with the extent of mitochondria impairment and the energy load in either cell line. Our data depict a scenario where the events related to the extrinsic pathway of apoptosis are the same in the two cell lines, the difference in the final outcome being related to the extent of mitochondria derangement, possibly due to the different ability of the cells to counteract ion homeostasis impairment.
- Subjects :
- Protein Folding
Programmed cell death
Amyloid
Necrosis
Apoptosis
Protein aggregation
Biochemistry
3T3 cells
Membrane Potentials
Mice
Adenosine Triphosphate
Bacterial Proteins
Cell Line, Tumor
Genetics
medicine
Animals
Molecular Biology
Caspase
Cell Death
biology
Chemistry
Escherichia coli Proteins
Proteins
Mitochondria
Cell biology
Enzyme Activation
medicine.anatomical_structure
Ion homeostasis
Cell culture
Caspases
Carboxyl and Carbamoyl Transferases
NIH 3T3 Cells
biology.protein
Tumor Suppressor Protein p53
medicine.symptom
Carrier Proteins
BH3 Interacting Domain Death Agonist Protein
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....f5c4791f10d42b3cd7c7a80c43db8191