Back to Search Start Over

Inverse relationship between connexin43 and desmin expression in cultured porcine aortic smooth muscle cells

Authors :
Nicholas J. Severs
Horst Robenek
Yu-Shien Ko
Gabriele Plenz
Source :
European journal of cell biology. 78(9)
Publication Year :
1999

Abstract

Our previous work has shown that in vascular tissues the elastic medial regions express high levels of the gap junctional protein, connexin43, but low levels of desmin, while the muscular medial regions express low levels of connexin43 but high levels of desmin. It is uncertain, however, whether this regional difference at the tissue level extends down to the level of the individual cell, or reflects an averaged relationship of groups of cells of different connexin43 and desmin expression. The present study has addressed this question using cultured porcine aortic smooth muscle cells. Immunoconfocal microscopic analysis of single-labeled cells showed that while smooth muscle alpha-actin, calponin and vimentin were positively labeled in the majority of medial smooth muscle cells both in intact porcine aorta and corresponding cultured cells, desmin and connexin43 labeling was highly heterogeneous. In the cultured cells, 0.3-0.5% of cells were found to be desmin-positive, and quantitative analysis after double labeling for desmin and connexin43 revealed that the desmin-positive cells were smaller, and contained significantly lower numbers and smaller sizes of connexin43 gap-junctional spots than did desmin-negative cells. Our findings demonstrate that an inverse expression pattern of connexin43 and desmin holds true at the level of the individual cell. This suggests a close relationship between intrinsic phenotypic control and the regulation of connexin43 expression in the arterial smooth muscle cell.

Details

ISSN :
01719335
Volume :
78
Issue :
9
Database :
OpenAIRE
Journal :
European journal of cell biology
Accession number :
edsair.doi.dedup.....f5b5f49ef61440afe170368f3637cfdc