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Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM)
- Source :
- PLoS ONE, Vol 14, Iss 10, p e0223794 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria. Here, the protein content of lipid rafts in Escherichia coli was explored by mass spectrometry analyses of Detergent Resistant Membranes (DRM). We report that at least three of the four E. coli flotillin homologous proteins were found to reside in DRM, along with 77 more proteins. Moreover, the proteomic data were validated by subcellular localization, using immunoblot assays and fluorescence microscopy of selected proteins. Our results confirm the existence of lipid raft-like microdomains in the inner membrane of E. coli and represent the first comprehensive profiling of proteins in these bacterial membrane platforms.
- Subjects :
- Proteomics
Signal peptide
Lipoproteins
Science
Cell Membranes
medicine.disease_cause
Biochemistry
Mass Spectrometry
Transmembrane Transport Proteins
03 medical and health sciences
Membrane Microdomains
Escherichia coli
medicine
Inner membrane
Integral Membrane Proteins
Post-Translational Modification
Integral membrane protein
Lipid raft
030304 developmental biology
0303 health sciences
Multidisciplinary
Chemistry
Escherichia coli Proteins
030302 biochemistry & molecular biology
Membrane Proteins
Biology and Life Sciences
Proteins
Cell Biology
Outer Membrane Proteins
Subcellular localization
Lipids
Membrane
Membrane protein
Multigene Family
Medicine
lipids (amino acids, peptides, and proteins)
Cellular Structures and Organelles
Signal Peptides
Chromatography, Liquid
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....f5b2b328e7c6b955240ad0c1cebdc33c