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Low-Affinity Nerve Growth Factor Receptor p75NTR Immunoreactivity in the Myocardium with Sympathetic Hyperinnervation
- Source :
- Journal of Cardiovascular Electrophysiology. 15:430-437
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- Introduction: We previously demonstrated the relationship between sympathetic nerve density in myocardium and the occurrences of ventricular arrhythmia. Nerve growth factor (NGF) regulates myocardial sympathetic innervation. However, it is unclear whether the NGF high-affinity receptor tyrosine kinase A (TrkA) and the NGF low-affinity receptor p75NTR are altered in the state of sympathetic hyperinnervation in the heart. The aim of this study was to determine the density and location of TrkA and p75NTR in canine ventricles with sympathetic hyperinnervation. Methods and Results: Myocardial sympathetic hyperinnervation was induced by local infusion of NGF into myocardium or left stellate ganglia, or chronic subthreshold electric stimulation to the left stellate ganglia. The results showed that TrkA immunoreactivity was absent in the myocardium. Low-affinity receptor p75NTR immunoreactivity was present in axons, Schwann cells, and interstitial cells of sympathetic nerves, as well as in interstitial cells of the myocardium. The density of p75NTR immunolabeled myocardial interstitial cells at the NGF infusion site was lower than that at the site remote from NGF infusion, yet the sympathetic nerve density was higher at the infusion site than the remote area. The density of p75NTR also was lower in the myocardium with high sympathetic nerve density, induced by NGF infusion or chronic electric stimulation of the left stellate ganglia, compared to control groups. Conclusion: The data indicate that p75NTR may be the main NGF receptor in the myocardium, and p75NTR immunopositive interstitial cells may have a role in regulating sympathetic nerve growth in canine heart. (J Cardiovasc Electrophysiol, Vol. 15, pp. 430-437, April 2004)
- Subjects :
- medicine.medical_specialty
Sympathetic Nervous System
Sympathetic nerve
Tropomyosin receptor kinase A
Receptor, Nerve Growth Factor
Infusion Site
Receptor tyrosine kinase
Dogs
Physiology (medical)
Internal medicine
Nerve Growth Factor
Animals
Low-affinity nerve growth factor receptor
Medicine
Receptor, trkA
Receptor
Infusion Pumps
NGF Receptor
biology
business.industry
Myocardium
Heart
Control Groups
Immunohistochemistry
Death, Sudden, Cardiac
Endocrinology
Nerve growth factor
nervous system
biology.protein
sense organs
Cardiology and Cardiovascular Medicine
business
Subjects
Details
- ISSN :
- 15408167 and 10453873
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Journal of Cardiovascular Electrophysiology
- Accession number :
- edsair.doi.dedup.....f5b23897e99c9e9f03032bccb46efbff
- Full Text :
- https://doi.org/10.1046/j.1540-8167.2004.03517.x