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Low-Affinity Nerve Growth Factor Receptor p75NTR Immunoreactivity in the Myocardium with Sympathetic Hyperinnervation

Authors :
B A Kai Chien
Che-Ming Chang
Hrayr S. Karagueuzian
B A Johnny Yi
Ji-Min Cao
Lan S. Chen
Ignacio Gonzalez-Gomez
Shengmei Zhou
Yasushi Miyauchi
Moshe Swissa
Peng-Sheng Chen
Kamlesh Asotra
Michael C. Fishbein
Katherine J. Fu
Source :
Journal of Cardiovascular Electrophysiology. 15:430-437
Publication Year :
2004
Publisher :
Wiley, 2004.

Abstract

Introduction: We previously demonstrated the relationship between sympathetic nerve density in myocardium and the occurrences of ventricular arrhythmia. Nerve growth factor (NGF) regulates myocardial sympathetic innervation. However, it is unclear whether the NGF high-affinity receptor tyrosine kinase A (TrkA) and the NGF low-affinity receptor p75NTR are altered in the state of sympathetic hyperinnervation in the heart. The aim of this study was to determine the density and location of TrkA and p75NTR in canine ventricles with sympathetic hyperinnervation. Methods and Results: Myocardial sympathetic hyperinnervation was induced by local infusion of NGF into myocardium or left stellate ganglia, or chronic subthreshold electric stimulation to the left stellate ganglia. The results showed that TrkA immunoreactivity was absent in the myocardium. Low-affinity receptor p75NTR immunoreactivity was present in axons, Schwann cells, and interstitial cells of sympathetic nerves, as well as in interstitial cells of the myocardium. The density of p75NTR immunolabeled myocardial interstitial cells at the NGF infusion site was lower than that at the site remote from NGF infusion, yet the sympathetic nerve density was higher at the infusion site than the remote area. The density of p75NTR also was lower in the myocardium with high sympathetic nerve density, induced by NGF infusion or chronic electric stimulation of the left stellate ganglia, compared to control groups. Conclusion: The data indicate that p75NTR may be the main NGF receptor in the myocardium, and p75NTR immunopositive interstitial cells may have a role in regulating sympathetic nerve growth in canine heart. (J Cardiovasc Electrophysiol, Vol. 15, pp. 430-437, April 2004)

Details

ISSN :
15408167 and 10453873
Volume :
15
Database :
OpenAIRE
Journal :
Journal of Cardiovascular Electrophysiology
Accession number :
edsair.doi.dedup.....f5b23897e99c9e9f03032bccb46efbff
Full Text :
https://doi.org/10.1046/j.1540-8167.2004.03517.x