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Integrating in silico with in vivo approach to investigate phthalate and bisphenol A mixture-linked asthma development: Positive probiotic intervention
- Source :
- Food and Chemical Toxicology
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The aim of this study was to explore the mechanisms of bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) mixture-induced asthma development and test probiotic as a potential positive intervention. Comparative Toxicogenomics Database (CTD) and ToppGene Suite were used as the main tools for in silico analysis. In vivo 28-day experiment was conducted on rats - seven groups (n = 6): (1) Control: corn oil, (2) P: probiotic (8.78 * 108 CFU/kg/day); (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day; (6) MIX: DEHP + DBP + BPA; (7) MIX + P. Lungs, thymus and kidneys were extracted and prepared for redox status and essential metals analysis. By conducting additional in vitro experiment, probiotic phthalate and BPA binding ability was explored. There were 24 DEHP, DBP and BPA asthma-related genes, indicating the three most probable mechanisms - apoptosis, inflammation and oxidative stress. In vivo experiment confirmed that significant changes in redox status/essential metal parameters were either prominent, or only present in the MIX group, indicating possible additive effects. In vitro experiment confirmed the ability of the multy-strain probiotic to bind DEHP/DBP/BPA mixture, while probiotic administration ameliorated mixture-induced changes in rat tissue.
- Subjects :
- Male
endocrine system
Bisphenol A
Dibutyl phthalate
Phthalic Acids
Thymus Gland
Endocrine Disruptors
010501 environmental sciences
Pharmacology
Probiotic
Kidney
Toxicology
medicine.disease_cause
Toxicogenetics
01 natural sciences
law.invention
03 medical and health sciences
chemistry.chemical_compound
Phenols
In vivo
law
Mechanisms
medicine
Animals
Humans
Computer Simulation
Benzhydryl Compounds
Toxicogenomic data mining
Endocrine disruptors
Lung
030304 developmental biology
0105 earth and related environmental sciences
0303 health sciences
Probiotics
Phthalate
General Medicine
Asthma
Rats
3. Good health
Oxidative Stress
chemistry
Toxicogenomics
Corn oil
Oxidative stress
Food Science
Subjects
Details
- ISSN :
- 02786915
- Volume :
- 158
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology
- Accession number :
- edsair.doi.dedup.....f5aa98668cab5072fed6024eb4807b8d