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Leptin-deficient ob/ob mice exhibit periodontitis phenotype and altered oral microbiome
- Source :
- Journal of Periodontal Research, 58(2), 392-402. Blackwell Munksgaard, Li, Z, Zheng, Z, Pathak, J L, Li, H, Wu, G, Xu, S, Wang, T, Cheng, H, Piao, Z, Jaspers, R T & Wu, L 2023, ' Leptin-deficient ob/ob mice exhibit periodontitis phenotype and altered oral microbiome ', Journal of Periodontal Research, vol. 58, no. 2, pp. 392-402 . https://doi.org/10.1111/jre.13099
- Publication Year :
- 2023
-
Abstract
- Background and Objective: Leptin-deficient obesity is associated with various systemic diseases including diabetes and low bone mass phenotype. However, the periodontal status of leptin-deficient obese individuals is still unclear. In this study, we aimed to analyze the periodontal status, alveolar bone phenotype, and oral microbiome status in leptin-deficient obese mice (ob/ob mice). Methods: This study used 12-week-old wild-type and ob/ob male mice. The alveolar bone phenotype and periodontal status in the maxilla were analyzed by micro-CT and histological analysis. Osteoclasts in alveolar bone were visualized by TRAP staining. Expressions of inflammatory markers (MMP-9, IL-1β, and TGF-β1) and osteoclastogenic markers (RANKL and OPG) in periodontium were analyzed by immunohistochemistry and RT-qPCR. The oral microbiome was analyzed by 16 S rDNA sequencing. Results: CEJ-ABC distance in maxillary molars (M1-M3) of ob/ob mice was significantly higher compared with that of wild-type. The alveolar bone BV/TV ratio was reduced in ob/ob mice compared with wild-type. Higher numbers of osteoclasts were observed in ob/ob mice alveolar bone adjacent to the molar root. Epithelial hyperplasia in gingiva and disordered periodontal ligaments was observed in ob/ob mice. RANKL/OPG expression ratio was increased in ob/ob mice compared with wild-type. Expressions of inflammatory markers MMP-9, IL-1β, and TGF-β1 were increased in ob/ob mice compared with wild-type. Oral microbiome analysis showed that beneficial bacteria Akkermansia and Ruminococcaceae_UCG_014 were more abundant in the wild-type mice while the inflammation-related Flavobacterium was more abundant in ob/ob mice. Conclusion: In conclusion, ob/ob mice showed higher expressions of inflammatory factors, increased alveolar bone loss, lower abundance of the beneficial bacteria, and higher abundance of inflammatory bacteria in the oral cavity, suggesting leptin-deficient obesity as a risk factor for periodontitis development in ob/ob mice.
Details
- Language :
- English
- ISSN :
- 00223484
- Volume :
- 58
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Periodontal Research
- Accession number :
- edsair.doi.dedup.....f573386c47d9a24035f0a61642cb51bc