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The Oncolytic Caprine Herpesvirus 1 (CpHV-1) Induces Apoptosis and Synergizes with Cisplatin in Mesothelioma Cell Lines: A New Potential Virotherapy Approach
- Source :
- Viruses; Volume 13; Issue 12; Pages: 2458, Viruses, Viruses, Vol 13, Iss 2458, p 2458 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Malignant mesothelioma (MM) is an aggressive asbestos-related cancer, against which no curative modalities exist. Oncolytic virotherapy is a promising therapeutic approach, for which MM is an ideal candidate; indeed, the pleural location provides direct access for the intra-tumoral injection of oncolytic viruses (OVs). Some non-human OVs offer advantages over human OVs, including the non-pathogenicity in humans and the absence of pre-existing immunity. We previously showed that caprine herpesvirus 1 (CpHV-1), a non-pathogenic virus for humans, can kill different human cancer cell lines. Here, we assessed CpHV-1 effects on MM (NCI-H28, MSTO, NCI-H2052) and non-tumor mesothelial (MET-5A) cells. We found that CpHV-1 reduced cell viability and clonogenic potential in all MM cell lines without affecting non-tumor cells, in which, indeed, we did not detect intracellular viral DNA after treatment. In particular, CpHV-1 induced MM cell apoptosis and accumulation in G0/G1 or S cell cycle phases. Moreover, CpHV-1 strongly synergized with cisplatin, the drug currently used in MM chemotherapy, and this agent combination did not affect normal mesothelial cells. Although further studies are required to elucidate the mechanisms underlying the selective CpHV-1 action on MM cells, our data suggest that the CpHV-1-cisplatin combination could be a feasible strategy against MM.
- Subjects :
- Oncolytic Virotherapy
caprine herpesvirus 1 (CpHV-1)
oncolytic virus (OV)
Cell Survival
Mesothelioma, Malignant
apoptosis
cisplatin
Antineoplastic Agents
Microbiology
Combined Modality Therapy
QR1-502
Article
malignant mesothelioma (MM)
Oncolytic Viruses
Infectious Diseases
Virology
synergism
Cell Line, Tumor
Humans
Varicellovirus
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses; Volume 13; Issue 12; Pages: 2458
- Accession number :
- edsair.doi.dedup.....f57314142551f0e6d1ec6894eae3d33c
- Full Text :
- https://doi.org/10.3390/v13122458