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In vitro design of a novel lytic bacteriophage cocktail with therapeutic potential against organisms causing diabetic foot infections
- Source :
- Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos), Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação, instacron:RCAAP
- Publication Year :
- 2014
-
Abstract
- In patients with diabetes mellitus, foot infections pose a significant risk. These are complex infections commonly caused by Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii, all of which are potentially susceptible to bacteriophages. Here, we characterized five bacteriophages that we had determined previously to have antimicrobial and wound-healing potential in chronic S. aureus, P. aeruginosa and A. baumannii infections. Morphological and genetic features indicated that the bacteriophages were lytic members of the family Myoviridae or Podoviridae and did not harbour any known bacterial virulence genes. Combinations of the bacteriophages had broad host ranges for the different target bacterial species. The activity of the bacteriophages against planktonic cells revealed effective, early killing at 4 h, followed by bacterial regrowth to pre-treatment levels by 24 h. Using metabolic activity as a measure of cell viability within established biofilms, we found significant cell impairment following bacteriophage exposure. Repeated treatment every 4 h caused a further decrease in cell activity. The greatest effects on both planktonic and biofilm cells occurred at a bacteriophage : bacterium input multiplicity of 10. These studies on both planktonic cells and established biofilms allowed us to better evaluate the effects of a high input multiplicity and a multiple-dose treatment protocol, and the findings support further clinical development of bacteriophage therapy. supported by TechnoPhage S.A. and Tecnifar; Foundation for Science and Technology (PTDC/SAUMIC/122816/2010 – ‘Biofilms in diabetic foot: microbial virulence characterization and cross-talk of major isolates’); FCT PhD fellowship (SFRH/BD/72872/2010); FCT ‘Ciência 2007’ programme info:eu-repo/semantics/publishedVersion
- Subjects :
- Microbiology (medical)
Acinetobacter baumannii
Staphylococcus aureus
HSM MED
chemotherapy
medicine.disease_cause
Microbiology
Bacteriophage
03 medical and health sciences
Podoviridae
medicine
Humans
Bacteriophages
030304 developmental biology
0303 health sciences
biology
030306 microbiology
Pseudomonas aeruginosa
Biofilm
General Medicine
Antimicrobial agents
biology.organism_classification
Antimicrobial
Virology
Diabetic Foot
3. Good health
Lytic cycle
Biofilms
Subjects
Details
- ISSN :
- 14735644
- Volume :
- 63
- Issue :
- Pt 8
- Database :
- OpenAIRE
- Journal :
- Journal of medical microbiology
- Accession number :
- edsair.doi.dedup.....f57219d1fbc4e6cf746ee6ebe7e1586c