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One-pot fabrication of FRET-based fluorescent probe for detecting copper ion and sulfide anion in 100% aqueous media
- Source :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 177
- Publication Year :
- 2016
-
Abstract
- The design of effective tools for detecting copper ion (Cu2+) and sulfide anion (S2-) is of great importance due to the abnormal level of Cu2+ and S2- has been associated with an increase in risk of many diseases. Herein, we report on the fabrication of fluorescence resonance energy transfer (FRET) based fluorescent probe PF (PEI-FITC) for detecting Cu2+ and S2- in 100% aqueous media via a facile one-pot method by covalent linking fluorescein isothiocyanate (FITC) with branched-polyethylenimine (b-PEI). PF could selectively coordinate with Cu2+ among 10 metal ions to form PF-Cu2+ complex, resulting in fluorescence quenching through FRET mechanism. Furthermore, the in situ generated PF-Cu2+ complex can be used to selectively detect S2- based on the displacement approach, resulting in an off-on type sensing. There is no obvious interference from other anions, such as Cl-, NO3-, ClO4-, SO42-, HCO3-, CO32-, Br-, HPO42-, F- and S2O32-. In addition, PF was successfully used to determine Cu2+ and S2- in human serum and tap water samples. Therefore, the FRET-based probe PF may provide a new method for selective detection of multifarious analysts in biological and environmental applications, and even hold promise for application in more complicated systems.
- Subjects :
- In situ
Anions
Sulfide
Metal ions in aqueous solution
chemistry.chemical_element
Sulfides
010402 general chemistry
Photochemistry
01 natural sciences
Analytical Chemistry
Ion
Fluorescence Resonance Energy Transfer
Humans
Instrumentation
Spectroscopy
Fluorescent Dyes
chemistry.chemical_classification
010405 organic chemistry
Chemistry
Water
Hydrogen-Ion Concentration
Copper
Fluorescence
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Förster resonance energy transfer
Covalent bond
Subjects
Details
- ISSN :
- 18733557
- Volume :
- 177
- Database :
- OpenAIRE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Accession number :
- edsair.doi.dedup.....f54c96817de6f880fa951d0b09927e30