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Differential expression and regulation of a human transgene, HLA-B27, in mouse placental and embryonic cell lines
- Source :
- Molecular Human Reproduction. 4:817-825
- Publication Year :
- 1998
- Publisher :
- Oxford University Press (OUP), 1998.
-
Abstract
- Unlike other somatic cells, human placental trophoblast cells do not express the highly polymorphic HLA-A and HLA-B human leukocyte major histocompatibility antigens that would stimulate maternal immunological rejection of the fetus. To investigate mechanisms underlying cell lineage-specific expression, cell lines were generated from homozygous matings of HLA-B27 transgenic mice. Trophoblast cell lines were generated from gestation day 10 placentas and fibroblasts were cultured from gestation day 13/14 embryos. Polymerase chain reaction (PCR) readily identified HLA-B DNA in transgenic trophoblastic cells but specific mRNA was of low abundance, being detectable by reverse transcriptase PCR but not by Northern blot hybridization. HLA-B-specific protein in/on the trophoblast cells was undetectable by cell enzyme-linked immunosorbent assay and the protein was not induced by exposing the trophoblastic cells to interferon-gamma (IFN-gamma). Restricted expression was specific for the HLA-B transgene and its antigen; IFN-gamma-inducible endogenous H-2Db class I antigens were detectable on the trophoblast cells. In contrast to the trophoblastic cells, HLA-B27 transgenic fibroblasts expressed IFN-gamma-inducible HLA class I antigens as well as H-2Db antigens. Thus, the mechanism(s) regulating expression of the polymorphic HLA-B antigen in trophoblastic cells is gene-specific, IFN-gamma-resistant and operative at the level of transcription or immediate post-transcription.
- Subjects :
- Embryology
Somatic cell
Placenta
Mice, Transgenic
Human leukocyte antigen
Biology
Cell Line
Interferon-gamma
Mice
Antigen
Pregnancy
Genetics
HLA-B Antigens
medicine
Animals
Humans
RNA, Messenger
Transgenes
Histocompatibility Antigen H-2D
Molecular Biology
HLA-B27 Antigen
reproductive and urinary physiology
Homozygote
H-2 Antigens
Gene Expression Regulation, Developmental
Obstetrics and Gynecology
Trophoblast
Cell Biology
Fibroblasts
Embryo, Mammalian
Embryonic stem cell
Molecular biology
Trophoblasts
Mice, Inbred C57BL
Reverse transcription polymerase chain reaction
medicine.anatomical_structure
Reproductive Medicine
Cell culture
embryonic structures
Female
Developmental Biology
Subjects
Details
- ISSN :
- 14602407 and 13609947
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular Human Reproduction
- Accession number :
- edsair.doi.dedup.....f54792a58251fbdfad3e550bfeb08de1