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Structural insight into DNA recognition by bacterial transcriptional regulators of the SorC/DeoR family
- Source :
- Acta Crystallographica Section D Structural Biology. 77:1411-1424
- Publication Year :
- 2021
- Publisher :
- International Union of Crystallography (IUCr), 2021.
-
Abstract
- The SorC/DeoR family is a large family of bacterial transcription regulators that are involved in the control of carbohydrate metabolism and quorum sensing. To understand the structural basis of DNA recognition, structural studies of two functionally characterized SorC/DeoR family members from Bacillus subtilis were performed: the deoxyribonucleoside regulator bsDeoR and the central glycolytic genes regulator bsCggR. Each selected protein represents one of the subgroups that are recognized within the family. Crystal structures were determined of the N-terminal DNA-binding domains of bsDeoR and bsCggR in complex with DNA duplexes representing the minimal operator sequence at resolutions of 2.3 and 2.1 Å, respectively. While bsDeoRDBD contains a homeodomain-like HTH-type domain, bsCggRDBD contains a winged helix–turn–helix-type motif. Both proteins form C2-symmetric dimers that recognize two consecutive major grooves, and the protein–DNA interactions have been analyzed in detail. The crystal structures were used to model the interactions of the proteins with the full DNA operators, and a common mode of DNA recognition is proposed that is most likely to be shared by other members of the SorC/DeoR family.
- Subjects :
- Models, Molecular
Binding Sites
biology
Protein Conformation
Regulator
DNA
Bacillus subtilis
Computational biology
Crystallography, X-Ray
biology.organism_classification
DNA-Binding Proteins
chemistry.chemical_compound
Quorum sensing
Bacterial Proteins
chemistry
Structural Biology
Bacterial transcription
Gene
Dna recognition
Protein Binding
Subjects
Details
- ISSN :
- 20597983
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica Section D Structural Biology
- Accession number :
- edsair.doi.dedup.....f4f3dc4e50c5a15b2e6c2063329d4e9b