Back to Search
Start Over
Countrywide spread of OXA-48 carbapenemase in Lebanon: surveillance and genetic characterization of carbapenem-non-susceptible Enterobacteriaceae in 10 hospitals over a one-year period
- Source :
- International Journal of Infectious Diseases, Vol 29, Iss C, Pp 139-144 (2014)
- Publication Year :
- 2014
- Publisher :
- Elsevier, 2014.
-
Abstract
- Summary Objectives To detect, characterize, and assess the genetic clonality of carbapenem-non-susceptible Enterobacteriaceae in 10 Lebanese hospitals in 2012. Methods Selected Enterobacteriaceae isolates with reduced susceptibility to carbapenems were subject to phenotypic study including antibiotic susceptibility, cloxacillin effect, modified Hodge test, and activity of efflux pump inhibitor. Carbapenemase genes were detected using PCR; clonal relatedness was studied by pulsed field gel electrophoresis. Results Out of 8717 Enterobacteriaceae isolated in 2012, 102 (1.2%) showed reduced susceptibility to carbapenems. Thirty-one (70%) of the 44 studied clinical isolates harbored bla OXA-48 , including 15 Klebsiella pneumoniae , eight Escherichia coli , four Serratia marcescens , three Enterobacter cloacae , and one Morganella morganii . The majority of OXA-48 producers co-secreted an extended-spectrum beta-lactamase, while one had an acquired AmpC of the ACC type. In the non-OXA-48 producers, carbapenem resistance was attributed to the production of acquired AmpC cephalosporinases of MOX or CIT type, outer membrane impermeability, and/or efflux pump overproduction. DNA fingerprints revealed that OXA-48 producers were different, except for clonal relatedness among four K. pneumoniae , two E. coli , two E. cloacae , and three S. marcescens . Conclusions Nosocomial carbapenem-non-susceptible Enterobacteriaceae are moderately spread in Lebanon and the predominant mechanism is OXA-48 production.
- Subjects :
- Microbiology (medical)
Carbapenem
Klebsiella pneumoniae
Carbapenem resistance
medicine.medical_treatment
beta-Lactamases
Microbiology
lcsh:Infectious and parasitic diseases
Bacterial Proteins
Enterobacteriaceae
Drug Resistance, Bacterial
Pulsed-field gel electrophoresis
medicine
Escherichia coli
polycyclic compounds
Humans
AmpC
lcsh:RC109-216
Lebanon
OXA-48
biology
General Medicine
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Hospitals
Anti-Bacterial Agents
Infectious Diseases
Carbapenems
Serratia marcescens
Beta-lactamase
bacteria
Morganella morganii
Enterobacter cloacae
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 18783511 and 12019712
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- International Journal of Infectious Diseases
- Accession number :
- edsair.doi.dedup.....f4c82ca62eb7ba6a11da501ba8475bb1