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A genetically encoded fluorescent reporter of ATP:ADP ratio
- Source :
- Nature methods
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- We constructed a fluorescent sensor of adenylate nucleotides by combining a circularly permuted variant of GFP with a bacterial regulatory protein, GlnK1, from Methanococcus jannaschii. The sensor's affinity for Mg-ATP was
- Subjects :
- DNA, Bacterial
Methanococcus
Archaeal Proteins
Adenylate kinase
Biosensing Techniques
Polymerase Chain Reaction
Biochemistry
Article
Green fluorescent protein
03 medical and health sciences
chemistry.chemical_compound
Adenosine Triphosphate
0302 clinical medicine
Bacterial Proteins
Nucleotide
Binding site
Molecular Biology
Fluorescent Dyes
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Binding Sites
biology
Cell Biology
biology.organism_classification
Adenosine Diphosphate
Luminescent Proteins
Adenosine diphosphate
Microscopy, Fluorescence
chemistry
ATP–ADP translocase
Adenosine triphosphate
030217 neurology & neurosurgery
Biotechnology
Subjects
Details
- ISSN :
- 15487105 and 15487091
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Nature Methods
- Accession number :
- edsair.doi.dedup.....f4c43db72d45194e7fefbb497675bebb
- Full Text :
- https://doi.org/10.1038/nmeth.1288