Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives

Authors :: Stewart Smith
Cairnan R. E. Duffy
Steve Pells
Heidi K. Mjoseng
Srinivas Velugotla
Ronald Pethig
Paul A. De Sousa
Source :: Velugotla, S, Pells, S, Mjoseng, H K, Duffy, C R E, Smith, S, de Sousa, P & Pethig, R 2012, ' Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives ', Biomicrofluidics, vol. 6, no. 4, 044113 . https://doi.org/10.1063/1.4771316
Publication Year :: 2012
Abstract: Assessment of the dielectrophoresis (DEP) cross-over frequency (fxo), cell diameter, and derivative membrane capacitance (Cm) values for a group of undifferentiated human embryonic stem cell (hESC) lines (H1, H9, RCM1, RH1), and for a transgenic subclone of H1 (T8) revealed that hESC lines could not be discriminated on their mean fxo and Cm values, the latter of which ranged from 14 to 20 mF/m2. Differentiation of H1 and H9 to a mesenchymal stem cell-like phenotype resulted in similar significant increases in mean Cm values to 41–49 mF/m2 in both lines (p Cm value to 28 mF/m2 (p

Language :: English
ISSN :: 19321058
Database :: OpenAIRE
Journal :: Velugotla, S, Pells, S, Mjoseng, H K, Duffy, C R E, Smith, S, de Sousa, P & Pethig, R 2012, ' Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives ', Biomicrofluidics, vol. 6, no. 4, 044113 . https://doi.org/10.1063/1.4771316
Accession number :: edsair.doi.dedup.....f4c2df8e1e2cf468328273c2d25335e1

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