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Characterization of cell-bound papain-soluble beta-lactamases in BRO-1 and BRO-2 producing strains of Moraxella (Branhamella) catarrhalis and Moraxella nonliquefaciens
- Source :
- European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 11(4)
- Publication Year :
- 1992
-
Abstract
- In Moraxella (Branhamella) catarrhalis and Moraxella nonliquefaciens strains isolated from clinical specimens in the south of Sweden two variants of beta-lactamase were distinguished by isoelectric focusing (IEF). The BRO-1 (Ravasio type) enzyme was the most common in Branhamella catarrhalis, constituting about 90% of the beta-lactamase found in this species, while the BRO-2 enzyme (1908 type) was as common as BRO-1 in Moraxella nonliquefaciens. The determinants mediating the production of BRO-1 and BRO-2 were both transferable by conjugation. Cell-bound beta-lactamase from reference strains producing BRO-1 and BRO-2 could be solubilized by papain digestion. The isoelectric point of the solubilized enzymes differed distinctly between BRO-1 (pI 6.5) and BRO-2 (pI 6.9). The molecular species of BRO-1 and BRO-2 released by papain digestion were purified by affinity chromatography with phenylboronic acid agarose gel. They had identical molecular weights of approximately 28,000. Their kinetic constants were indistinguishable for a number of substrates and beta-lactamase inhibitors.
- Subjects :
- Microbiology (medical)
Microbial Sensitivity Tests
Biology
beta-Lactams
Moraxella nonliquefaciens
Chromatography, Affinity
beta-Lactamases
chemistry.chemical_compound
Affinity chromatography
Papain
Moraxella
Isoelectric Point
Electrophoresis, Agar Gel
Sweden
Molecular mass
Isoelectric focusing
General Medicine
biology.organism_classification
Anti-Bacterial Agents
Molecular Weight
Kinetics
Infectious Diseases
Isoelectric point
chemistry
Biochemistry
Conjugation, Genetic
Branhamella
Agarose
Moraxella catarrhalis
Subjects
Details
- ISSN :
- 09349723
- Volume :
- 11
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....f4b32e0a5f3b1992469cd1076866db19