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Thymoquinone alleviates nonalcoholic fatty liver disease in rats via suppression of oxidative stress, inflammation, apoptosis
- Source :
- Naunyn-Schmiedeberg's Archives of Pharmacology. 389:381-391
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Nonalcoholic steatohepatitis (NAFLD) is a progressive form of liver disease that leads to advanced fibrosis. The present study was designed to assess the hepatoprotective effect of thymoquinone (TQ) on liver functions, insulin resistance, and PPAR-γ expression in NAFLD. Rats were divided into two main groups: one fed with normal rat chow diet and the other with high-fat high-cholesterol diet group for 6 weeks. Every group was subdivided into three subgroups (n = 8): treated with saline, low dose TQ (10 mg/kg), high dose TQ (20 mg/kg). High fat high cholesterol diet caused marked liver damage as noted in histopathology and significant increase in liver index, liver enzymes. There was significant increase in the insulin resistance, serum cholesterol, triglyceride, PPAR-γ gene overexpression with significant decrease in HDL. Additionally, oxidative stress increased by measuring MDA associated with significant decrease in serum total antioxidant capacity. As markers of inflammation, hepatic TNF-α was significantly increased with decrease in IL10. Further, there was increase in BAX protein with decrease in Bcl as compared to control group. This model of 6 weeks high-fat high-cholesterol diet showed minimal fibrosis as noticed by increase MMP2 and Masson trichrome satin. Co-treatment with TQ improved all previous parameters. High dose was more effective, although mostly non-statistically significant. TQ may have a promising agent to improve hepatic steatosis, oxidative stress; inflammatory, apoptotic status, fibrosis and so prevent liver damage in patients with NAFLD. Although PPAR-γ was significantly under-expressed by TQ, insulin resistance was improved significantly suggesting a role of liver damage.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Anti-Inflammatory Agents
Apoptosis
Diet, High-Fat
Liver Cirrhosis, Experimental
medicine.disease_cause
Antioxidants
Hepatitis
03 medical and health sciences
chemistry.chemical_compound
Liver disease
Insulin resistance
Non-alcoholic Fatty Liver Disease
Fibrosis
Internal medicine
Nonalcoholic fatty liver disease
Benzoquinones
medicine
Animals
Rats, Wistar
Thymoquinone
bcl-2-Associated X Protein
Pharmacology
Triglyceride
Tumor Necrosis Factor-alpha
business.industry
General Medicine
medicine.disease
Lipids
Interleukin-10
PPAR gamma
Oxidative Stress
030104 developmental biology
Endocrinology
Liver
Proto-Oncogene Proteins c-bcl-2
chemistry
Inflammation Mediators
Insulin Resistance
Steatosis
business
Oxidative stress
Subjects
Details
- ISSN :
- 14321912 and 00281298
- Volume :
- 389
- Database :
- OpenAIRE
- Journal :
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Accession number :
- edsair.doi.dedup.....f4b061cbe9f887fbefebc8851f5a9dda