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miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration
- Source :
- BioMed Research International, BioMed Research International, Vol 2018 (2018)
- Publication Year :
- 2018
- Publisher :
- Hindawi Limited, 2018.
-
Abstract
- Osteoporosis is a systemic bone metabolic disease that is highly prevalent in the elderly population, particularly in postmenopausal women, which results in enhanced bone fragility and an increased susceptibility to fractures. However, the underlying molecular pathogenesis mechanisms still remain to be further elucidated. In this study, in a rat ovariectomy- (OVX-) induced postmenopausal osteoporosis model, aberrant expression of a microRNA miR-142-5p and vascular cell adhesion molecule 1 (VCAM-1) was found by RNA sequencing analysis and qRT-PCR. Using a dual-luciferase reporter assay, we found that miR-142-5p can bind to and decrease expression of VCAM-1 mRNA. Such reduction was prohibited when the miR-142-5p binding site in VCAM-1 3'UTR was deleted, and Western blotting analyses validated the fact that miR-142-5p inhibited the expression ofVCAM-1 protein. Bone marrow-derived mesenchymal stem cells (BMMSCs) transfected with miR-142-5p showed a significantly decreased migration ability in a Transwell migration assay. Collectively, these data indicated the important role of miR-142-5p in osteoporosis development involving targeting VCAM-1 and inhibiting BMMSC migration. Refereed/Peer-reviewed
- Subjects :
- 0301 basic medicine
Article Subject
Osteoporosis
lcsh:Medicine
Vascular Cell Adhesion Molecule-1
bone metabolic disease
Bone Marrow Cells
General Biochemistry, Genetics and Molecular Biology
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Cell Movement
microRNA
medicine
Animals
VCAM-1
3' Untranslated Regions
Migration Assay
General Immunology and Microbiology
Cell adhesion molecule
lcsh:R
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell migration
General Medicine
medicine.disease
osteoporosis
Rats
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
chemistry
inhibiting BMMSC migration
Cancer research
Female
Bone marrow
Research Article
Subjects
Details
- ISSN :
- 23146141 and 23146133
- Volume :
- 2018
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....f498ef9113b7a964bf15904fbdc9a447