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miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration

Authors :
Yun Zhu
Shen Xu
Xueguan Xie
Cory J. Xian
Yugang Liu
Xingbo Hu
Xiongwen Zhang
Qiang Na
Zhaowei Teng
Jianping Liu
Guojun Wei
Xiguang Zhang
Teng, Zhaowei
Xie, Xueguan
Zhu, Yun
Liu, Jianping
Hu, Xingbo
Na, Qiang
Zhang, Xiongwen
Wei, Guojun
Xu, Shen
Liu, Yugang
Zhang, Xiguang
Xian, Cory J
Source :
BioMed Research International, BioMed Research International, Vol 2018 (2018)
Publication Year :
2018
Publisher :
Hindawi Limited, 2018.

Abstract

Osteoporosis is a systemic bone metabolic disease that is highly prevalent in the elderly population, particularly in postmenopausal women, which results in enhanced bone fragility and an increased susceptibility to fractures. However, the underlying molecular pathogenesis mechanisms still remain to be further elucidated. In this study, in a rat ovariectomy- (OVX-) induced postmenopausal osteoporosis model, aberrant expression of a microRNA miR-142-5p and vascular cell adhesion molecule 1 (VCAM-1) was found by RNA sequencing analysis and qRT-PCR. Using a dual-luciferase reporter assay, we found that miR-142-5p can bind to and decrease expression of VCAM-1 mRNA. Such reduction was prohibited when the miR-142-5p binding site in VCAM-1 3'UTR was deleted, and Western blotting analyses validated the fact that miR-142-5p inhibited the expression ofVCAM-1 protein. Bone marrow-derived mesenchymal stem cells (BMMSCs) transfected with miR-142-5p showed a significantly decreased migration ability in a Transwell migration assay. Collectively, these data indicated the important role of miR-142-5p in osteoporosis development involving targeting VCAM-1 and inhibiting BMMSC migration. Refereed/Peer-reviewed

Details

ISSN :
23146141 and 23146133
Volume :
2018
Database :
OpenAIRE
Journal :
BioMed Research International
Accession number :
edsair.doi.dedup.....f498ef9113b7a964bf15904fbdc9a447