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Cell cycle-dependent expression of gamma-tubulin in the amicronuclear ciliate Tetrahymena pyriformis
- Source :
- Protist. 158(1)
- Publication Year :
- 2006
-
Abstract
- In ciliates, different microtubular structures are nucleated from diverse Microtubule Organizing Centers (MTOCs). gamma-Tubulin is a tubulin superfamily member that plays an essential role in microtubule nucleation at the MTOCs. However, little is known about mechanisms regulating the activity of gamma-tubulin on different MTOCs and during the cell cycle. In Tetrahymena thermophila, the alpha- and beta-tubulin expression is regulated mainly at the transcriptional level, and changes in the ratio of polymerized/unpolymerized tubulin dimers lead to an increase or decrease of alpha- and beta-tubulin transcription. This study deals with the characterization of gamma-tubulin in the amicronuclear ciliate Tetrahymena pyriformis. Sequence analysis revealed some specific substitutions in nucleotide-binding loops characteristic of the Tetrahymena genus and putative conserved phosphorylation sites located on the external surface of the gamma-tubulin molecule. gamma-Tubulin expression during the cell cycle, in the presence of microtubular poisons and after deciliation, was also characterized. We found that gamma-tubulin mRNA levels are correlated with basal body proliferation and gamma-tubulin nuclear localization. We also found that gamma-tubulin expression changes during anti-microtubular drugs treatment, but does not changes during reciliation. These findings suggest a relationship between the level of unpolymerized tubulin dimers and gamma-tubulin transcription.
- Subjects :
- Models, Molecular
Tetrahymena pyriformis
Cell Cycle
Molecular Sequence Data
Tetrahymena
Protozoan Proteins
macromolecular substances
Sequence Analysis, DNA
Cell cycle
Biology
DNA, Protozoan
biology.organism_classification
Microbiology
Cell biology
Tubulin
Gene Expression Regulation
Microtubule
biology.protein
Basal body
Animals
Cytoskeleton
Microtubule nucleation
Subjects
Details
- ISSN :
- 14344610
- Volume :
- 158
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Protist
- Accession number :
- edsair.doi.dedup.....f495a7070b5ad8f49e592aed4270ea93