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Cytokeratin 8 interacts with clumping factor B: a new possible virulence factor target
- Source :
- Microbiology. 156:3710-3721
- Publication Year :
- 2010
- Publisher :
- Microbiology Society, 2010.
-
Abstract
- Staphylococcus aureusis a human pathogen of growing clinical significance, owing to its increasing levels of resistance to most antibiotics. Infections range from mild wound infections to severe infections such as endocarditis, osteomyelitis and septic shock. Adherence ofS. aureusto human host cells is an important step, leading to colonization and infection. Adherence is mediated by a multiplicity of proteins expressed on the bacterial surface, including clumping factor B. In this study, we aimed to identify new targets of clumping factor B in human keratinocytes by undertaking a genome-wide yeast two-hybrid screen of a human keratinocyte cDNA library. We show that clumping factor B is capable of binding cytokeratin 8 (CK8), a type II cytokeratin. Using a domain-mapping strategy we identified amino acids 437–464 as necessary for this interaction. Recombinantly expressed fragments of both proteins were used in pull-down experiments and confirmed the yeast two-hybrid studies. Analysis withS. aureusstrain Newman deficient in clumping factor B showed the clumping factor B-dependence of the interaction with CK8. We postulate that the clumping factor B–CK8 interaction is a novel factor inS. aureusinfections.
- Subjects :
- Keratinocytes
Staphylococcus aureus
Virulence Factors
Molecular Sequence Data
Virulence
Biology
medicine.disease_cause
Microbiology
Virulence factor
Cell Line
Bacterial genetics
Cytokeratin
Two-Hybrid System Techniques
medicine
Humans
Amino Acid Sequence
Adhesins, Bacterial
cDNA library
Keratin-8
Staphylococcal Infections
Molecular biology
Clumping factor A
Bacterial adhesin
Protein Binding
Subjects
Details
- ISSN :
- 14652080 and 13500872
- Volume :
- 156
- Database :
- OpenAIRE
- Journal :
- Microbiology
- Accession number :
- edsair.doi.dedup.....f47b5afde7e320b0b6704aeb367a94e6