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Transcriptome profiling reveals phase-specific gene expression in the developing barley inflorescence
- Source :
- Crop Journal, Vol 8, Iss 1, Pp 71-86 (2020)
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The shape of an inflorescence varies among cereals, ranging from a highly branched panicle in rice to a much more compact spike in barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.). However, little is known about the molecular basis of cereal inflorescence architecture. We profiled transcriptomes at three developmental stages of the barley main shoot apex — spikelet initiation, floral organ differentiation, and floral organ growth — and compared them with those from vegetative seedling tissue. Transcript analyses identified 3688 genes differentially transcribed between the three meristem stages, with a further 1394 genes preferentially expressed in reproductive compared with vegetative tissue. Co-expression assembly and Gene Ontology analysis classified these 4888 genes into 28 clusters, revealing distinct patterns for genes such as transcription factors, histone modification, and cell-cycle progression specific for each stage of inflorescence development. We also compared expression patterns of VRS (SIX-ROWED SPIKE) genes and auxin-, gibberellic acid- and cytokinin-associated genes between two-rowed and six-rowed barley to describe regulators of lateral spikelet fertility. Our findings reveal barley inflorescence phase-specific gene expression, identify new candidate genes that regulate barley meristem activities and flower development, and provide a new genetic resource for further dissection of the molecular mechanisms of spike development. Keywords: Inflorescence meristem, Transcriptome, Gene expression, Hormones, Barley
- Subjects :
- 2. Zero hunger
0106 biological sciences
Genetics
0303 health sciences
Candidate gene
fungi
lcsh:S
food and beverages
Plant Science
Meristem
Biology
lcsh:S1-972
01 natural sciences
lcsh:Agriculture
Transcriptome
03 medical and health sciences
Inflorescence
Gene expression
Hordeum vulgare
lcsh:Agriculture (General)
Agronomy and Crop Science
Gene
030304 developmental biology
010606 plant biology & botany
Panicle
Subjects
Details
- ISSN :
- 22145141
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- The Crop Journal
- Accession number :
- edsair.doi.dedup.....f47a6fdc7f0ea56a6e3c43cf7a540f35