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Penicillium chrysogenum polypeptide extract protects tobacco plants from tobacco mosaic virus infection through modulation of ABA biosynthesis and callose priming
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- Fungal polypeptide-induced callose priming restricts the cell-to-cell movement of tobacco mosaic virus in N. benthamiana via the ABA biosynthetic pathway.<br />The polypeptide extract of the dry mycelium of Penicillium chrysogenum (PDMP) can protect tobacco plants from tobacco mosaic virus (TMV), although the mechanism underlying PDMP-mediated TMV resistance remains unknown. In our study, we analysed a potential mechanism via RNA sequencing (RNA-seq) and found that the abscisic acid (ABA) biosynthetic pathway and β-1,3-glucanase, a callose-degrading enzyme, might play an important role in PDMP-induced priming of resistance to TMV. To test our hypothesis, we successfully generated a Nicotiana benthamiana ABA biosynthesis mutant and evaluated the role of the ABA pathway in PDMP-induced callose deposition during resistance to TMV infection. Our results suggested that PDMP can induce callose priming to defend against TMV movement. PDMP inhibited TMV movement by increasing callose deposition around plasmodesmata, but this phenomenon did not occur in the ABA biosynthesis mutant; moreover, these effects of PDMP on callose deposition could be rescued by treatment with exogenous ABA. Our results suggested that callose deposition around plasmodesmata in wild-type plants is mainly responsible for the restriction of TMV movement during the PDMP-induced defensive response to TMV infection, and that ABA biosynthesis apparently plays a crucial role in PDMP-induced callose priming for enhancing defence against TMV.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
viruses
Mutant
Priming (immunology)
Nicotiana benthamiana
Plant Science
Plasmodesma
Penicillium chrysogenum
01 natural sciences
tobacco mosaic virus (TMV)
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Tobacco
PDMP
Tobacco mosaic virus
priming
Glucans
Abscisic acid
Plant Diseases
biology
AcademicSubjects/SCI01210
plasmodesmata
Plant Extracts
Chemistry
N. benthamiana
fungi
Callose
food and beverages
Cell Biology
Plants, Genetically Modified
biology.organism_classification
Research Papers
infection
Cell biology
Tobacco Mosaic Virus
030104 developmental biology
ABA
Peptides
callose
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....f473e6b1f872854bb406e2d79a7d9e21