Back to Search
Start Over
T cell-intrinsic S1PR1 regulates endogenous effector T-cell egress dynamics from lymph nodes during infection
- Source :
- Proceedings of the National Academy of Sciences. 113:2182-2187
- Publication Year :
- 2016
- Publisher :
- Proceedings of the National Academy of Sciences, 2016.
-
Abstract
- Viral clearance requires effector T-cell egress from the draining lymph node (dLN). The mechanisms that regulate the complex process of effector T-cell egress from the dLN after infection are poorly understood. Here, we visualized endogenous pathogen-specific effector T-cell migration within, and from, the dLN. We used an inducible mouse model with a temporally disrupted sphingosine-1-phosphate receptor-1 (S1PR1) gene specifically in endogenous effector T cells. Early after infection, WT and S1PR1(-/-) effector T cells localized exclusively within the paracortex. This localization in the paracortex by CD8 T cells was followed by intranodal migration by both WT and S1PR1(-/-) T cells to positions adjacent to both cortical and medullary lymphatic sinuses where the T cells exhibited intense probing behavior. However, in contrast to WT, S1PR1(-/-) effector T cells failed to enter the sinuses. We demonstrate that, even when LN retention signals such as CC chemokine receptor 7 (CCR7) are down-regulated, T cell intrinsic S1PR1 is the master regulator of effector T-cell emigration from the dLN.
- Subjects :
- 0301 basic medicine
T-Lymphocytes
T cell
Mice, Transgenic
C-C chemokine receptor type 7
Biology
Infections
Lymphocyte Activation
Vesicular stomatitis Indiana virus
Mice
Mice, Congenic
03 medical and health sciences
0302 clinical medicine
Cell Movement
medicine
Animals
Cytotoxic T cell
Sphingosine-1-Phosphate Receptors
Lymph node
S1PR1
Mice, Knockout
Multidisciplinary
Effector
Endothelial Cells
Biological Sciences
Cell biology
Mice, Inbred C57BL
Receptors, Lysosphingolipid
030104 developmental biology
medicine.anatomical_structure
Lymphatic system
030220 oncology & carcinogenesis
Immunology
Lymph Nodes
Vesicular Stomatitis
CC chemokine receptors
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 113
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....f46e31fa76dd335bf6d99c29bdc03644