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Genome-wide analysis of differentially expressed genes and the modulation of PEDV infection in Vero E6 cells
- Source :
- Microbial Pathogenesis
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- PEDV remains one of the most important swine diseases that infects pigs of all ages. It causes devastating viral enteric disease in piglets with a high mortality rate, leading to significant threats and huge economic loss to the pork industry. In this study, a transcriptomic shotgun sequencing (RNA-Seq) procedure was used to study gene responses against PEDV infection. Genome-wide analysis of differentially expressed genes (DEGs) was performed in Vero E6 cells post-PEDV infection. mTOR signaling pathway activator-MHY1485, and inhibitor-PP242 were used to study the antiviral function. Results revealed that the IRF3 was significantly up-regulated post-PEDV infection. Although most of the IFN-regulatory and –related genes evaluated in this study were either down-regulated or remained unchanged, IL11 behaved significantly up-regulated, with the peak at 16 hpi. Nearly 90% of PEDV infections were suppressed in the PP242 pretreated cells whereas the reverse effect was observed in the MYH1485 pretreated cells. Results indicated that the mTOR signaling pathway played a vital role in the PEDV antiviral regulation in the Vero E6 cells. Future studies will contribute to better understand the cellular antiviral mechanism against PEDV.<br />Highlights • RNA-Seq was used to study gene responses against PEDV infection. • Genome-wide analysis of DEGs was performed in Vero E6 cells post-PEDV infection. • The mTOR signaling pathway activator and inhibitor can affect the PEDV infection rate.
- Subjects :
- Proteomics
0301 basic medicine
Indoles
Proteome
Swine
Morpholines
Down-Regulation
Gene Expression
RNA-Seq
Biology
Virus Replication
Antiviral Agents
Microbiology
Article
Transcriptome
03 medical and health sciences
Chlorocebus aethiops
Animals
Antiviral
Vero Cells
Gene
Swine Diseases
030102 biochemistry & molecular biology
Triazines
Shotgun sequencing
Gene Expression Profiling
Porcine epidemic diarrhea virus
PEDV
Interleukin-11
Virology
030104 developmental biology
Infectious Diseases
Differentially expressed genes
mTOR signaling pathway
Purines
Vero cell
Coronavirus Infections
IRF3
Function (biology)
Signal Transduction
Subjects
Details
- ISSN :
- 08824010
- Volume :
- 117
- Database :
- OpenAIRE
- Journal :
- Microbial Pathogenesis
- Accession number :
- edsair.doi.dedup.....f464101907304125f296968e2441df22
- Full Text :
- https://doi.org/10.1016/j.micpath.2018.02.004