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A Simple and Inexpensive Microinjection System for Zebrafish Embryos and Larvae
- Source :
- Zebrafish. 14:581-585
- Publication Year :
- 2017
- Publisher :
- Mary Ann Liebert Inc, 2017.
-
Abstract
- Microinjection is a widely used technique to inject defined volumes and concentrations of substances and explore their physiological function in vivo. The technique has been particularly successful with zebrafish embryos; however, the injection equipment can be relatively expensive and therefore available only to well-funded laboratories. In this study, a simple, cheap, easy-to-assemble, and easy-to-use setup with a straightforward, accurate, and efficacious calibration method is introduced. The accuracy of this calibration method was tested by comparing with the results of calibration methods that are currently used in high-cost systems. Injection success with this low-cost system was verified based on the presence of injected dyes in zebrafish embryos, the absence of any significant morphological and behavioral differences between 3,4,-dichloroaniline-treated and untreated embryos, and larval viability.
- Subjects :
- Injection equipment
021110 strategic, defence & security studies
Physiological function
Aniline Compounds
Embryo, Nonmammalian
Microinjections
0211 other engineering and technologies
02 engineering and technology
Anatomy
Biology
021001 nanoscience & nanotechnology
biology.organism_classification
In vivo
Larva
Zebrafish embryo
Animals
Animal Science and Zoology
Coloring Agents
0210 nano-technology
Zebrafish
Microinjection
Developmental Biology
Biomedical engineering
Subjects
Details
- ISSN :
- 15578542 and 15458547
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Zebrafish
- Accession number :
- edsair.doi.dedup.....f45d36f810143f0f6cd6c600d39f1b60