Back to Search
Start Over
Protective effect of sarsasapogenin in TNBS induced ulcerative colitis in rats associated with downregulation of pro-inflammatory mediators and oxidative stress
- Source :
- Immunopharmacology and Immunotoxicology. 43:571-583
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- Ulcerative colitis (UC) is a chronic inflammatory bowel condition considered by oxido-nitrosative stress and the release of pro-inflammatory cytokines that affects the mucosal lining of the colon. Sarsasapogenin (SG), as an active component, has been found in many plants, and it exhibits potential protective effects, such as anti-inflammatory, antioxidant, anti-psoriasis, anti-arthritis, anti-asthma, anti-depressant and anti-cancer. However, the effects of SG on UC remain unknown.The purpose of this study was to investigate the effects of SG on 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced UC in rats.Thirty Wistar rats were randomized into five groups: (i) Normal control, (ii) Disease control (TNBS), (iii) Sarsasapogenin (SG) (50 µg/rat), (iv) Fluticasone (FC) (50 µg/rat), (v) Sarsasapogenin + Fluticasone (SG + FC) (25 µg/rat). UC was induced in rats by trans-rectal instillation of TNBS (10 mg/kg). SG, FC and SG + FC were administered for 11 days and on the 8th day colitis was induced. Several molecular, biochemical and histological alterations were evaluated in the colon tissue. All treatment group results were compared to the TNBS group results.The study results revealed that treatment of rats with SG and SG + FC combination significantly decreased the colon weight/length ratio, macroscopic inflammation score, lesions score, diarrhea score and adhesion score. Combination treatment in rats significantly reduced the production of biochemical parameters, proinflammatory cytokines, haematological parameters, serum IgE levels and restored the oxidative stress markers. SG and SG + FC treatment also considerably restored the histopathological changes induced by TNBS.Thus, SG and SG + FC combination could alter the disease progression and could be a hopeful therapeutic target for the management of UC by reducing its dose in combination with FC to elude the long term adverse effects of FC.
- Subjects :
- Male
Immunology
Anti-Inflammatory Agents
Down-Regulation
Toxicology
medicine.disease_cause
Antioxidants
Proinflammatory cytokine
chemistry.chemical_compound
Downregulation and upregulation
Spirostans
medicine
Animals
Immunology and Allergy
Rats, Wistar
Fluticasone
Pharmacology
business.industry
General Medicine
Sarsasapogenin
medicine.disease
Ulcerative colitis
digestive system diseases
Rats
Oxidative Stress
Trinitrobenzenesulfonic Acid
chemistry
Cytoprotection
Colitis, Ulcerative
Inflammation Mediators
business
Oxidative stress
Drugs, Chinese Herbal
medicine.drug
Subjects
Details
- ISSN :
- 15322513 and 08923973
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Immunopharmacology and Immunotoxicology
- Accession number :
- edsair.doi.dedup.....f4554c31a6c24d8ba6e8422e1b80805f
- Full Text :
- https://doi.org/10.1080/08923973.2021.1955919