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Cell-free DNA and circulating tumor cell kinetics in a pre-clinical head and neck Cancer model undergoing radiation therapy
- Source :
- BMC Cancer, Vol 21, Iss 1, Pp 1-11 (2021), BMC Cancer
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Background Monitoring circulating tumor DNA (ctDNA) and circulating tumor cells (CTCs), known as liquid biopsies, continue to be developed as diagnostic and prognostic markers for a wide variety of cancer indications, mainly due to their minimally invasive nature and ability to offer a wide range of phenotypic and genetic information. While liquid biopsies maintain significant promising benefits, there is still limited information regarding the kinetics of ctDNA and CTCs following radiation therapy which remains a vital treatment modality in head and neck cancers. This study aims to describe the kinetics of ctDNA and CTCs following radiation exposure in a preclinical rabbit model with VX2 induced buccal carcinoma. Methods Seven rabbits were inoculated with VX2 cells in the buccal mucosa and subjected to radiation. At selected time points, blood sampling was performed to monitor differing levels of ctDNA and CTC. Plasma ctDNA was measured with quantitative PCR for papillomavirus E6 while CTCs were quantified using an immunomagnetic nanoparticles within a microfluidic device. Comparisons of CTC detection with EpCAM compared to multiple surface markers (EGFR, HER2 and PSMA) was evaluated and correlated with the tumor size. Results Plasma ctDNA reflects the overall tumor burden within the animal model. Analysis of correlations between ctDNA with tumor and lymph node volumes showed a positive correlation (R = 0.452 and R = 0.433 [p Conclusion This study demonstrates the utility of ctDNA and CTCs detection in response to radiation treatment in a preclinical head and neck model, allowing for better understanding of liquid biopsy applications in both clinical practice and research development.
- Subjects :
- Male
Cancer Research
medicine.medical_treatment
Rabbit
Cottontail rabbit papillomavirus
Open Reading Frames
Circulating tumor cell
Biomarkers, Tumor
Genetics
Carcinoma
medicine
Animals
Liquid biopsy
Head and neck cancer
Lymph node
RC254-282
Circulating tumor DNA
Radiation
VX2
Immunomagnetic Separation
business.industry
Liquid Biopsy
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Cancer
Radiotherapy Dosage
Epithelial Cell Adhesion Molecule
medicine.disease
Tumor Burden
Radiation therapy
medicine.anatomical_structure
Oncology
Head and Neck Neoplasms
Carcinoma, Squamous Cell
Cancer research
Nanoparticles
Mouth Neoplasms
Oral cavity cancer preclinical model
Rabbits
business
Cell-Free Nucleic Acids
Neoplasm Transplantation
Research Article
Blood sampling
Subjects
Details
- ISSN :
- 14712407
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....f4374cf12098ef2454b88b7dd0ecebd3